乳腺肿瘤干细胞分选的相关研究  

Sorting methods of breast cancer stem cells

作　　者：郭崇勇[1] 宋科瑛[1] 李克[1] 

机构地区：[1]上海交通大学附属第一人民医院普外科,200080

出　　处：《国际肿瘤学杂志》2011年第5期358-361,共4页Journal of International Oncology

摘　　要：“肿瘤干细胞”学说的提出为肿瘤包括乳腺肿瘤在内的治疗提供了新的思路。乳腺肿瘤干细胞正确的分选纯化是研究的关键，其分选主要历经侧群细胞分选、无血清悬浮培养、细胞表面分子标记分选、ALDEFLUOR试剂盒检测乙醛脱氢酶1高活性分选法4个阶段；由于化疗会富集肿瘤干细胞，部分学者把化疗耐药也作为一种分选的方法。目前有关乳腺肿瘤干细胞分选结果的争论主要集中在标志物CD44＋CD24-、ALDH1＋之间。哪种分选方法或标志物的选择更适宜分选乳腺肿瘤干细胞成为亟待解决的问题。In recent years, the theory of cancer stem cells has provided a new perspective on the treatment of cancers including breast cancer. The accurate sorting of breast cancer stem cells is critical. The sorting procedure is consist of 4 steps： isolation of side population, serumree suspension culture, determination of specific cell surface markers and of the activity of aldehyde dehydrogenase 1 （ ALDH1 ） enzymatic through the ALDEFLUOR assay. Some studies choose drug resistance as an additional method for sorting cancer stem cells because of the enrichment of cancer stem ceils after chemotherapy. The controversy about the sorting outcome of breast cancer stem cell mainly focus on molecular markers like CD44＋CD24 - and ALDH1＋ The problem needed to be settled is to identify which sorting method and markers are appropriate.

关 键 词：肿瘤干细胞 乳腺肿瘤 抗药性 肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]