空间植物组织培养的优化方法探讨  

Study on Optimization Methods in Plant Tissue Culture in Space

作　　者：张岳[1] 郑慧琼[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所

出　　处：《载人航天》2011年第3期58-64,共7页Manned Spaceflight

摘　　要：空间微重力环境中将生物材料培养好是确保空间实验成功的一个重要前提。采用空间实验模拟培养盒和实验室常用的培养皿进行拟南芥愈伤组织的优化方法研究。实验结果表明:相对密闭的小环境中,氧气供应不足是实验细胞生长发育的主要限制因素,在培养基中添加0.002mg/ml硝酸银和1%活性炭可以明显提高细胞对缺氧的耐受性。In the microgravity environment in space, it is a prerequisite for the success of experiment to keep the biological samples growing well. An optimization study on the Arabidopsis callus is carried out using experiment containers and regular Petri dishes. The result shows that in a relatively small and closed culture chambers, shortage of oxygen supply is a major limiting factor to the growth and development of the cells, and it can greatly enhance the tolerance of cells to oxygen shortage, when the culture medium containes 0.002 mg/ml silver nitrate and 1% activated carbon.

关 键 词：空间实验 植物组织培养 拟南芥 

分 类 号：V419.6[航空宇航科学与技术—航空宇航推进理论与工程]