重症肝炎患者肠道分泌片和紧密连接蛋白的变化  

Change of intestinal secretory component and tight junction proteins in severe hepatitis

作　　者：刘冬妍[1] 邓纯博[1] 刘沛[2] 

机构地区：[1]中国医科大学附属盛京医院实验研究中心(卫生部小儿先天畸形重点实验室),辽宁沈阳110004 [2]中国医科大学第一附属医院传染科,辽宁沈阳110001

出　　处：《中国现代医学杂志》2011年第11期1289-1291,1296,共4页China Journal of Modern Medicine

基　　金：国家自然基金项目(No:30871158);辽宁省教委课题(No:2008783)

摘　　要：目的研究重症肝炎患者肠道分泌片(SC)和紧密连接蛋白(ZO-1、Occludin)的变化,了解重症肝炎患者伴发腹部症状的原因。方法采用免疫组织化学方法分别检测肠道SC和ZO-1、Occludin。结果与对照组比较,重症肝炎患者肠道SC和ZO-1、Occludin染色明显减弱。对照组光密度SC为(9.47±3.84),ZO-1为(1.08±0.35),Occludin为(12.08±8.06);重症肝炎患者光密度SC为(0.59±0.10),ZO-1为(0.41±0.30),Oc-cludin为(2.92±1.37),与对照组比较,重症肝炎患者SC、ZO-1和Occludin光密度显著减少(P<0.01)。结论重症肝炎患者肠道SC和ZO-1、Occludin表达下降,导致肠道屏障功能发生异常,这是引起腹部症状的原由之一。【Objective】To study the change of intestinal secretory component（SC） and tight junction pro-teins（ZO-1,Occludin） in severe hepatitis and to understand the reason of severe hepatitis patients accompa-nied by abdominal symptom.【Methods】 SC and tight junction proteins of gut were assayed by immunohisto-chemistry.【Results】 Compared with the control group,the staining of SC and tight junction proteins-ZO-1 and occludin in severe hepatitis group were notably decrease.The SC OD value was（9.47 ± 3.84）,ZO-1 OD value was（1.08 ± 0.35）,Occludin OD value was（12.08 ± 8.06） in control group,and in severe hepatitis group the SC OD value was（0.59 ± 0.1）,ZO-1 OD value was（0.41 ± 0.30）,Occludin OD value was（2.92 ± 1.37）.【Conclusions】In severe hepatitis SC and tight junction proteins-ZO-1,Occludin expression of gut decreased and lead to the abnormality of barrier of gut.This is one of the reasons of resulting in abdominal symptom in severe hepatitis.

关 键 词：重症肝炎 紧密连接蛋白 分泌片 

分 类 号：R575.3[医药卫生—消化系统]