应用表面加强激光解析电离-飞行时间质谱技术建立非活动性HBsAg携带者血清蛋白质谱筛选模型  

Detection of serum markers by SELDI-TOF-MS protein chip technology in non-active hepatitis B s' antigen carriers

作　　者：邓敬桓[1] 李山[1] 张志勇[2] 韦小敏[2] 陈智平[2] 秦雪[1] 刘银坤[3] 

机构地区：[1]广西医科大学第一附属医院临床实验部,广西南宁530021 [2]广西医科大学公共卫生学院,广西南宁530021 [3]复旦大学生物医学研究院肝癌研究中心,上海200032

出　　处：《中国现代医学杂志》2011年第11期1346-1349,1361,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金资助项目(No:30760243);广西研究生教育创新课题(No:2008105981002M219);广西大型仪器协作共用网资助

摘　　要：目的比较非活动性HBsAg携带者和健康人的血清蛋白标记物并建立相关筛选模型。方法用表面加强激光解析电离-飞行时间质谱技术(SELDI-TOF-MS)及CM10蛋白芯片对35例非活动性HBsAg携带者、35例健康人血清样本进行分析,将获得的血清蛋白质指纹图谱用计算机软件进行比较分析,建立非活动性HBsAg携带者的比较筛选模型。结果非活动性HBsAg携带者组与健康人对照组共有28个差异有统计学意义的蛋白峰;建立诊断模型的灵敏度94%(33/35),特异度80%(28/35),准确率为87%(61/70)。结论建立的血清蛋白质指纹图谱模型能够区分非活动性HBsAg携带者与健康人,SELDI技术在诊断及特异性血液生物标志分子的筛选等方面具有较好的临床应用价值。【Objective】To screen relatively specific markers in serum from healthy people and non-active hepatitis B s＇ antigen carriers.【Methods】SELDI-TOF-MS Protein Chip was used to detect serum protein patterns of 35 non-active hepatitis B s antigen carriers and 35 healthy people,diagnostic model was developed and validated by using BPS.【Results】At the molecular weight range from 2000 to 50000,28 protein peaks were obviously differ-ent between non-active hepatitis B carriers and the healthy people.Analyzed by BPS,The diagnostic model separat-ed the non-active hepatitis B carriers from the control samples with accuracy of 87%（28/35）,sensitivity of 94%（33/ 35）and specificity of 80%（28/35）.【Conclusion】SELDI-TOF-MS Protein Chip array technology has better clinical application value in diagnosis and detection of specific serum biomarkers.

关 键 词：非活动性慢性无症状乙型肝炎病毒携带者 表面加强激光解析电离-飞行时间质谱 血清蛋白标记物 

分 类 号：R575.1[医药卫生—消化系统]