耐有机溶剂脂肪酶产生菌的筛选和鉴定  

The Screening and Identification of a Novel Strain Producing an Organic Solvent-Tolerant Lipase

作　　者：彭仁[1,2] 林金萍[1] 魏东芝[1] 

机构地区：[1]华东理工大学反应器工程国家重点实验室,上海200237 [2]江西师范大学生命科学学院,江西南昌330022

出　　处：《江西师范大学学报（自然科学版）》2011年第2期197-200,共4页Journal of Jiangxi Normal University(Natural Science Edition)

基　　金：国家重点基础研究发展计划(2009CB724703);江西省科技支撑计划(200800361)资助项目

摘　　要：从国内各地采取土样分离筛选产耐有机溶剂脂肪酶的新菌株,通过形态和生理生化特征以及系统进化树分析,筛选到的新菌株命名为Pseudomonas aeruginosa CS-2.来自Pseudomonas aeruginosa CS-2的粗脂肪酶液在乙腈中酶活提高,在苯、氯仿、正己烷、石油醚和异辛烷中表现出较高的稳定性.A novel strain which produced an organic solvent-tolerant lipase was isolated from soils in different areas of China.The strain was identified as Pseudomonas aeruginosa CS-2 by morphological,physiological and biochemical features and phylogenetic tree analysis.The crude lipase was activated in the presence of acetonitrile and it exhibited high stability in presence of benzene,chloroform,n-Hexane,petroleum ether and isooctane.

关 键 词：铜绿假单胞菌 有机溶剂耐受性 脂肪酶 

分 类 号：Q93[生物学—微生物学]