生防细菌L5生防相关因子的初步分析及其种类鉴定  

Characterization and identification of a biocontrol bacterial strain L5

作　　者：钱兰娟[1] 李倩[1] 张清霞[1] 童蕴慧[1] 徐敬友[1] 

机构地区：[1]扬州大学园艺与植物保护学院,扬州225009

出　　处：《植物病理学报》2011年第3期295-300,共6页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(C140601-31000875);江苏省高校自然基金资助项目(09KJB210005);扬州大学大学生科技创新基金资助项目

摘　　要：从扬州郊区土壤中分离到细菌菌株L5,该菌株抑菌谱广,对多种病原真菌都有明显的拮抗能力,离体叶片生物测定结果表明菌株L5对番茄灰霉病防效达72%。对该菌株的生防相关性状进行分析,结果表明菌株L5可产生硝吡咯菌素(Pyrrolnitrin)和嗜铁素等抗菌物质。经过16S rDNA序列、16S-23S rDNA间区序列同源性分析和生理生化性状的测定,将该菌株鉴定为气味沙雷氏菌(Serratia odorifera)。细菌菌株L5包含2个16S-23S rDNA的间区(IGSs),其中IGS1内含tRNAGlu基因,间区类型属于IGSG;IGS2内含tRNAIle和tRNAAla基因,间区类型属于IGSIA。Bacterial strain L5 was isolated from soil in Yangzhou and showed inhibitory effect to many pathogenic fungi in dual-culture tests.This strain produced pyrrolnitrin and siderphore,and showed 72% efficiency to control grey mould caused by Botrytis cinerea on detached tomato leaves.The strain L5 was identified as Serratia odorifera based on sequence analysis of 16S rDNA and 16S-23S rDNA intergenic spacers（IGS）,physiological and biochemical characterization.The analysis of IGS sequence found two different types of IGS in strain L5.One included the tRNAGlu,and the other contained a cluster of tRNAIle and tRNAAla.

关 键 词：16S RDNA 16S-23S RDNA间区 沙雷氏菌 硝吡咯菌素 番茄灰霉病菌 

分 类 号：S154.39[农业科学—土壤学]