酸性α-淀粉酶高产菌株的原生质体诱变选育  

Screening and Breeding of High-yield Strains for Acid α-Amylase by UV Mutagenesis

作　　者：褚清龙[1] 刘新育[1] 吕向云[1] 陈红歌[1] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002

出　　处：《安徽农业科学》2011年第13期7701-7702,共2页Journal of Anhui Agricultural Sciences

基　　金：河南省教育厅自然科学研究计划项目(2010A180006)

摘　　要：[目的]以产酸性α-淀粉酶菌解淀粉芽孢杆菌B-5为出发菌株,通过对B-5原生质体进行紫外线诱变以达到提高产酸性α-淀粉酶活力的目的。[方法]在溶菌酶浓度为20 mg/ml,37℃酶解90 min条件下,原生质体制备率达到94%。然后经紫外线诱变处理,从中筛选水解圈与菌落比值较大者进行发酵,测定酸性α-淀粉酶活力。[结果]从大量突变菌株中筛选得到1株α-淀粉酶活力为267 U/ml的突变菌株UV-329,其产酶活力较出发菌株B-5提高了254.2%。[结论]利用紫外线对解淀粉芽孢杆菌B-5原生质体进行诱变是一种有效的微生物育种方法。[Objective] Bacillus amyloliquefaciens 5 was used as initial strain for preparation of protoplast and UV-mutagenesis in order to promote its acid α-amylase production.[Method] The preparation rate of protoplast could reach 94% with 20 mg/ml of lysozyme by enzymolysising for 90 min at 37 ℃.The mutant with the bigger ratio of hydrolyzation circle diameter to bacterial colony diameter was screened after UV mutagenesis so as to assay acid α-amylase activities.[Result] Mutant UV-329 with acid α-amylase activity of 267 U/ml was screened from a lot of mutants,with an increase of 254.2% over that of the initial strain.[Conclusion] UV mutagenesis for protoplast was an effective breeding method.

关 键 词：解淀粉芽孢杆菌 原生质体 诱变 酸性Α-淀粉酶 

分 类 号：Q939.97[生物学—微生物学]