鸡传染性法氏囊病病毒河南分离株HeYD VP2基因高变区基因克隆及序列分析  

Sequence Analysis of the VP2 Hypervariable Region of A Field Infectious Bursal Disease Virus Strain HeYD Isolated from Henan Province

作　　者：鄂巍[1,2] 张改平[1] 罗俊[2] 滕蔓[2] 王兴涛[2] 王爱萍[1] 

机构地区：[1]郑州大学生物工程系,河南郑州450001 [2]河南省农业科学院农业部动物免疫学重点开放实验室/河南省动物免疫学重点实验室,河南郑州450002

出　　处：《河南农业科学》2011年第3期149-153,共5页Journal of Henan Agricultural Sciences

基　　金：河南省基础与前沿计划项目(082300433201)

摘　　要：为初步确定新分离的鸡传染性法氏囊病毒(IBDV)河南株HeYD的毒力,根据GenBank数据库中已报道的IBDV基因组序列,设计合成了1对VP2基因高变区特异性引物,应用RT-PCR方法对分离自河南省某发病鸡场的IBDV野毒株HeYD的VP2基因高变区进行了基因克隆及序列分析,并与其他参考毒株高变区进行了序列比对。序列分析结果表明,HeYD株的VP2基因高变区具有IBDV超强毒株典型的氨基酸序列特征,即222位(A)、256位(I)、294位(I)和299位(S),表明HeYD为超强毒株。利用VP2基因高变区序列构建基因进化树,发现HeYD株与国内超强毒株如xin-1、GX8-99以及国外超强毒株如日本的OKYM和欧洲的DV86毒株亲缘关系较近。To identify the subtype of a field infectious bursal disease virus（IBDV） strain HeYD isolated from Henan province,a pair of specific primers were designed according to the genome sequences of IBDV previously reported for amplifying the cDNA of IBDV VP2 hypervariable region.The VP2 hypervariable region of HeYD was cloned by reverse transcription polymerase chain reaction（RT-PCR）,sequenced and compared with other reference IBDV strains.The results showed that the amino acid sequence of HeYD VP2 hypervariable region represents the typical feature of those of very virulent IBDV（vvIBDV） strains at the positions of 222（A）,256（I）,294（I）,and 299（S）.The phylogenetic tree based on the DNA sequences of IBDV VP2 hypervariable regions indicated that HeYD strain was far away from some of China vvIBDV stains,but close to other China vvIBDV strains such as xin-1,GX8-99,and some foreign vvIBDV strains such as OKYM and DV86.

关 键 词：传染性法氏囊病病毒 VP2基因高变区 基因克隆 基因进化树 

分 类 号：S852.65[农业科学—基础兽医学]