发酵性丝孢酵母产油脂中纤维质糖化液脱毒工艺对比及优化研究  

Comparison of detoxification technology and optimization production of lipid by Trichosporon fermentans in fiber-derived saccharified liquid

作　　者：冯冲[1,2] 王艳颖[1] 康静[3] 刘艳丽[2] 宋安东[1] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002 [2]中州大学实验管理中心,河南郑州450044 [3]新乡医学院生命科学技术系,河南新乡453003

出　　处：《北京化工大学学报（自然科学版）》2011年第3期87-91,共5页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：国家自然科学基金(70741032);国家农业成果转化资金项目(2006D00070597);河南省科技攻关重点项目(092102210110)

摘　　要：纤维质在进行稀酸水解糖化时,会产生一些对菌体发酵有抑制作用的物质。为了除去抑制物,提高纤维质糖化液的发酵产脂能力,对玉米秸秆稀酸水解液的脱毒产脂条件进行了优化,优化结果为:蒸煮20 min,活性炭用量为1.0 g/L,乙醛用量为0.4 g/L,黄孢原毛平革菌接种量(以孢子计)为4×108 L-1。此时,菌体生物量为21.2 g/L,油脂含量为50.9%,油脂质量浓度为10.8 g/L,糖油转化率为13.5%。When lignocellulosic biomass is hydrolyzed by dilute acid,it produces substances that inhibit bacterial fermentation.In order to remove fermentation inhibitors and improve the fermentability of saccharified fibers,the optimized conditions for detoxification and lipid production using cornstalk dilute acid hydrolysate have been studied.The results showed that heating for 20 min,adding activated carbon（1.0g/L） and acetaldehyde（0.4g/L）,and an inoculum size of Phanerochaete chrysosporium of 4×108 L-1 were the optimum conditions.Under these optimized culture conditions,the biomass and lipid content were 21.2g/L and 50.9%,respectively;the lipid concentration and lipid conversion rate of sugars were 10.8g/L and 13.5% respectively.

关 键 词：纤维质糖化液 脱毒 发酵 优化 

分 类 号：Q939.9[生物学—微生物学] TK6[动力工程及工程热物理—生物能]