芘荧光探针法结合曲线拟合研究胆盐-磷脂混合纳米胶束的聚集行为  

The Study of Aggregation Behavior of Bile Salt-phosphatidylcholine Mixed Nano-micelle by Pyrene Fluorescence Probe Spectrometry Methods and Curve Fitting

作　　者：陆国庆[1,2] 郑江[3] 郑新川[3] 鲁永玲[3] 何凤慈[1] 

机构地区：[1]中国人民解放军第三军医大学科研部,重庆400038 [2]中国人民解放军成都军区总医院临床药学科,四川成都610083 [3]中国人民解放军第三军医大学西南医院中心实验室,重庆400038

出　　处：《中国药业》2011年第10期21-23,共3页China Pharmaceuticals

摘　　要：目的研究不同磷脂比例的胆盐-磷脂混合纳米胶束的聚集行为。方法以芘为荧光探针,测定不同磷脂比例的胆盐-磷脂混合纳米胶束增溶芘后的荧光光谱,以I1/I3值(I1=372 nm,I3=383 nm)为纵坐标、胶束浓度对数值logC为横坐标,利用Origin8.0软件进行曲线拟合,以S型曲线的突变中点切线和平行切线的交点定义临界胶束浓度。结果磷脂/胆盐比例(W/W)为2∶1,1∶1,1∶2的混合胶束临界胶束浓度分别为0.034,0.048,0.055 g/L。结论提高混合胶束中磷脂比例,可以减小胶束的临界胶束浓度,有利于胶束发挥更大的增溶作用。Objective The aim of current study was to study the aggregation behavior of bile salt-phosphatidylcholine mixed nano-micelle prepared by different phospholipid fraction.Methods The fluorescence spectra of different phospholipid fraction of bile salt-phosphatidylcholine mixed nano-micelle were measured,in which pyrene was solubilized and used as fluorescence probe.I1/I3（I1=372 nm,I3=383 nm）,according to the curves of fluorescence spectra,was taken as Y-axis and logC as X-axis simultaneously.The plot of pyrene versus bile salt-phosphatidylcholine mixed nano-micelle was S-Curve drawn by Origin softwear 8.0 through curve fitting.The intersection point of two tangents to curve was considered as critical micelle concentration.Results The critical micelle concentration of bile salt-phosphatidylcholine mixed nano-micelle with different phospholipids fraction（2 ∶1,1 ∶1,1 ∶2） were respectively 0.034,0.048,0.055 g/L.Conclusion The results indicated that increasing phospholipid fraction had lower critical micelle concentration and better solubilization.

关 键 词：芘 荧光探针 胆盐 磷脂 临界胶束浓度 

分 类 号：R944.9[医药卫生—药剂学]