局部应用缓释免疫抑制剂对胰岛细胞移植的影响  

Local application of slow-release immunosuppressant in islet cell transplantation

作　　者：梁青山[1] 季洪亮[1] 孔庆柱[2] 孙向东[1] 李波[1] 冯景[1] 党少华[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院甲状腺乳腺外科,哈尔滨150001 [2]承德医学院附属医院骨四科,承德067000

出　　处：《国际外科学杂志》2011年第5期307-310,F0003,共5页International Journal of Surgery

摘　　要：目的 探索局部应用免疫抑制剂对提高移植胰岛细胞的存活率及改善全身不良反应的影响.方法 造模：将链脲霉素按200 mg/kg注射到昆明小鼠腹腔内,48、72 h后测血糖,连续测两次≥20 mol/L作为实验动物糖尿病型.腹腔内移植入胰岛细胞的量为8 000 IE/kg,环孢素用量为1.5 mg/（100 g·d）.实验分3组：（1）经胃灌注免疫抑制剂与腹腔内注射微囊化胰岛细胞;（2）单纯腹腔内注射微囊化胰岛细胞;（3）腹腔内注射载有免疫抑制剂的微囊化的活性炭颗粒与微囊化的胰岛细胞.检测移植后大鼠的血糖及病理的变化.结果 1组与3组血糖变化差异无统计学意义（P＞0.05）,与2组相比（P＞0.05）差异无统计学意义.局部应用免疫制剂后,胰岛细胞作用时间较对照组长,胰岛周嗣纤维化程度较轻,C肽水平明显高于单纯移植组.结论 与经胃灌注应用免疫抑制相比,局部应用缓慢释放免疫抑制剂的活性碳颗粒效果与之相当,但后者可延长移植胰岛细胞的作用时间并可减轻局部反应.Objective To explore the local application of immunosuppressant in improving the survival rate of the transplanted islet cells and systemic side effects.Methods The streptozocin of 200 ms/kg was injected into the abdominal cavity of the Wistar rats,the blood sugar was tested after 48,and 72 hours,and the rats with two consecutive measurements ≥20 mol/L were taken as the experimental animal model.The dose of pancreatic islet cells transplanted into the abdominal cavity was 8 000 IE,/kg,and that of cyclosporine dosage was 1.5 mg/（100 g·d）.The pancreatic islet cells were divided into three groups：（1）systemic immunosuppressive agents through stomach lavage with the intraperitoneal injection of microencapsulated islet cells;（2）pure intraperitoneal injection of microencapsulated islet cells;（3）intraperitoneal injection microencapsulated activated carbon particles loaded with immunosuppressants,and mieroencapsulated islet cells.Changes of blood glucose and pathological in rats after transplantation were detected.Results The blood glucose of group 3 and group 1 showed no significant difference（P〉0.05）,as well as compared with group 2（P〉0.05）.But the local application of immune agents could prolong the effective time of the islet cells and attenuate the fibrotic extent of the surrounding islets when compared with the control group,the C peptide level in applicating immunosuppressive agents group was significantly hisher in the immunosuppressive group than the pure transplantation group.Conclusion Compared with the systemic immune suppression via stomach lavage,local application of slow-release immunosuppressive agents showed the same effects of activated carbon particles,with a prolonged the effective time of islet cell and reduced topical side effects in the latter

关 键 词：胰岛 微囊化 活性炭 

分 类 号：R587.1[医药卫生—内分泌]