细胞单糖气相色谱图鉴别细菌的研究  

STUDY ON THE DISCRIMINATION OF BACTERIA BY GAS CHROMATOGRAPHIC PROFILES OF CELLULAR MONOSACCHARIDES

作　　者：周方[1] 朱厚础[2] 王令春[2] 高树德[1] 

机构地区：[1]军事医学科学院微生物学流行病学研究所 [2]军事医学科学院生物工程研究所

出　　处：《微生物学报》1990年第1期7-15,共9页Acta Microbiologica Sinica

基　　金：国家自然科学基金

摘　　要：本文介绍细胞单糖毛细管柱气相色谱图的绘制方法,对所得细菌细胞单糖气相色谱图中的某些组分进行分析鉴定,并借助电子计算机对细菌细胞单糖模式进行聚类分析。用上述方法鉴别了24株(5种)芽孢杆菌,均得到满意结果。结果表明,炭疽芽孢杆菌和蜡状芽孢杆菌的单糖图谱有较明显的区别。A procedure for obtaining gas charomato-graphic (GC) profiles of bacterial cellular mo-nosaccharides was described.Some of the un-known component peaks in these profiles wereidentified.And,based on the complete lin-kage cluster analysis with the Euclidean dis-tance coefficient,the interpretation of the re-sulting cellular monosaccharides of bacteriawere performed by mini-computer.By meansof this method,the discrimination of 5 species(24 strains) of aerobic endospore-forming bac-teria.The results showed that there were de-fined differences between the profiles of cel-lular monosaccharides of B.anthracis and B.cereus.This procedure has provided a usefulmethod for the classification and identifica-tion of microorganisms,for their physiologicaland biochemical studies,and for studies ontheir subcellular components.

关 键 词：细菌鉴定 细胞单糖 气相色谱 

分 类 号：Q93－331[生物学—微生物学]