AMD3100、CXCR7抗体对自身免疫性脑脊髓炎大鼠脊髓和脾细胞SDF1α、CXCR4、CXCR7 mRNA表达的影响  

Effects of AMD3100 and CXCR7 antibody on the expressions of SDF1α,CXCR4 and CXCR7 mRNA in spinal cord and splenocytes in EAE rats

作　　者：李冠宇[1] 王松[2] 季晓辉[2] 金庆文[1] 

机构地区：[1]南京医科大学第一附属医院神经内科,210029 [2]南京医科大学微生物与免疫学教研室,210029

出　　处：《中国神经免疫学和神经病学杂志》2011年第3期206-210,共5页Chinese Journal of Neuroimmunology and Neurology

基　　金：国家自然科学基金面上资助项目(30870125)

摘　　要：目的研究AMD3100、CXCR7抗体对实验性自身免疫性脑脊髓炎(experimental autoimmune en-cephalomyelitis,EAE)大鼠脊髓和脾脏中趋化因子SDF1α及其受体CXCR4、CXCR7表达的影响。方法采用MBP68-86和完全福氏佐剂配置的完全抗原免疫Lewis大鼠制备EAE模型。将大鼠随机分为对照组、AMD3100组、CXCR7抗体组和EAE组,于免疫后第0、2、4、6天给予相应药物干预:AMD3100组按体质量2.5mg/kg腹腔注射AMD3100,CXCR7抗体组腹腔注射CXCR7抗体20μg/只,EAE组腹腔注射等量生理盐水。每天对大鼠进行神经功能评分,免疫后第15天行RT-PCR检测各组脊髓及脾脏中SDF1α、CXCR4、CXCR7mRNA表达,行HE染色观察大鼠脊髓组织病理变化。结果 (1)与对照组相比,EAE组大鼠脊髓血管周围有大量性炎细胞浸润,神经功能评分明显增加,AMD3100组、CXCR7抗体组和EAE组脊髓SDF1α、CXCR4、CXCR7 mRNA表达增加(P<0.01),脾细胞SDF1α、CXCR7 mRNA表达减少(P<0.01);(2)与EAE组相比,AMD3100组大鼠神经功能评分增加,脾细胞CXCR4、CXCR7 mRNA表达增加(P<0.01),脊髓SDF1α、CXCR4、CXCR7表达无统计学差异;(3)与EAE组相比,CXCR7抗体组大鼠神经功能评分增加,脾细胞SDF1α、CXCR4、CXCR7 mRNA表达增加(P<0.01),脊髓SDF1α、CXCR4、CXCR7 mRNA表达无统计学差异。结论 AMD3100和CXCR7抗体均能加重EAE病情,上调EAE脾细胞CXCR4和CXCR7 mRNA的表达。Objective To investigate the relationship between intraperitoneal injection of AMD3100 and CXCR7 antibody and the clinic symptoms and the expressions of SDF1α mRNA, CXCR4 mRNA and CXCR7 mRNA in spinal cord and splenocytes in experimental autoimmune encephalomyelitis （EAE） rats. Methods EAE Lewis rats were induced by subcutaneous injection of MBP68-86 in complete Freund＇ s adjuvant, and the rats injected with physiological saline in complete Freund＇ s adjuvant were taken as controls. Thirty-six female Lewis rats were divided into four groups： control group （n= 8）, EAE group （n = 8）, administration of AMD3100 group （AMD3100, n= 8）, administration of CXCR7 antibody group （CXCR7Ab, n= 8）. The treatment groups of EAE rats received AMD3100 [ （2.5 mg/ （kg · 2 d）] or CXCR7Ab [20μg/each ＊ 2 d）], respectively, on the day 0, 2, 4 and 6 after immunization. The diet, rnovement and body weight of the rats were daily evaluated. RT-PCR was used to detect the expressions of SDF1α mRNA, CXCR4 mRNA and CXCR7 mRNA in spinal cord and splenocytes, and the brain and spinal cord were stained with hematoxylin eosin. Results （1） Compared with the controls, the EAE group showed increased parenchymal infiltration and perivaseular cuffing of mononuclear cells and increased neurological deficit scores. Took the expression of GAPDH as reference, the expressions of SDFla mRNA, CXCR4 mRNA and CXCR7 mRNA in spinal cord were higher in the EAE group than those in the control group （P 〈 0.01） , while SDF1α mRNA and CXCR7 mRNA expressions in splenocytes in the EAE group were lower than those in the control group （P〈0.01）. （2） Compared with the EAE group, the clinical signs was exacerbated and CXCR4 mRNA and CXCR7 mRNA expressions in splenocytes increased in AMD3100 group （P〈0.01）, with comparable expressions of SDF1α mRNA, CXCR4 mRNA and CXCR7 mRNA in spinal cord. （3） Compared with EAE group, CXCR7Ab group showed exacerbated clinical signs, and higher expressions of SDF1α mRNA,

关 键 词：脑脊髓炎 自身免疫性 实验性 多发性硬化 趋化因子SDF1 受体CXCR4 受体CXCR7 

分 类 号：R744.51[医药卫生—神经病学与精神病学]