吉西他滨和丝裂霉素联合应用对人肺癌细胞A549的作用  

The combination effect of gemcitabine with mitomycin on human lung cancer A549 cell line in vitro

作　　者：赵可新[1] 李军霞[2] 姜杉[1] 王伟刚[1] 胡建平[1] 单靖珊[1] 

机构地区：[1]中国石油天然气集团公司中心医院,河北省廊坊市065000 [2]河北医科大学药理学教研室

出　　处：《河北医药》2011年第9期1301-1304,共4页Hebei Medical Journal

摘　　要：目的观察吉西他滨和丝裂霉素联合应用对人肺癌细胞A549的抑制效应,并探讨其作用机制。方法用MTT法检测化疗药物对人肺癌细胞A549生长的抑制作用;流式细胞术(FCM)检测细胞周期分布和凋亡率。结果 (1)丝裂霉素和吉西他滨单独和联合用药可浓度依赖性抑制A549细胞的生长。(2)丝裂霉素和吉西他滨在合用72h后,当Fa>0.18时,丝裂霉素和吉西他滨合用指数CI均<1,两种药物之间是协同作用,当Fa<0.18时,合用指数CI大于1,两种药物之间为拮抗作用。(3)丝裂霉素和吉西他滨同时用药时抑制率最高,其次是先用丝裂霉素后用吉西他滨,抑制率最低是先用吉西他滨后用丝裂霉素。(4)丝裂霉素和不同浓度的吉西他滨合用,除与2.5μg/ml吉西他滨合用时CI>1外,其余均<1。10μg/ml吉西他滨和不同浓度的丝裂霉素合用,除与0.0625μg/ml合用时CI>1外,其余均<1。(5)丝裂霉素和吉西他滨单用和合用时对细胞周期和凋亡均有影响。结论丝裂霉素和吉西他滨单独和联合用药可浓度依赖性抑制A549细胞的生长。不同用药次序和药物浓度比例也影响药物联合作用。Objective To investigate the combination effect of gemcitabine with mitomycin on human lung cancer A549 cell line in vitro,and to explore its action mechanism.Methods MTT assay was used to analyze the inhibition effect of chemotherapy drugs on the growth of human lung cancer A549 cell line,and the cell cycle and cell apoptosis were detected by flow cytometry.Results The gemcitabine and mitomycin alone or combination application could inhibit the growth of human lung cancer A549 cell line in a time and concentration dependent mode.The combination index（CI） was less than 1 at 72h when Fa0.18,which indicated that gemcitabine and mitomycin had synergistic effect.However,the combination index was more than 1 at 72h when Fa0.18,which indicated that gemcitabine and mitomycin had antagonistic effect.The combination application of gemcitabine with mitomycin could get the highest inhibitory rate,next,the moderate inhibitory rate was got when mitomycin was given prior to gemcitabine,and the lowest inhibitory rate was got when gemcitabine was given prior to mitocymin.The CI was all less than 1 when mitomycin was combined with gemcitabine at different concentrations.The gemcitabine and mitomycin alone or combination application had influence on the cell cycle and cell apoptosis.Conclusion The gemcitabine and mitocymin alone or in combination application can inhibit the growth of human lung cancer A549 cell line in a time-and concentration-dependent manner.The different drug concentrations and medication succession may affect the synergistic effect of the drugs.

关 键 词：吉西他滨 丝裂霉素 人肺癌A549 中效原理 

分 类 号：R730.5[医药卫生—肿瘤] R734.2[医药卫生—临床医学]