金黄色葡萄球菌与难养菌协同共凝集法检测苹果锈果病  

BACTERIA-STAPHYLOCOCCUS CO-AGGLUTINATION TEST (SA-TEST) FOR DETECTION OF FASTIDIOUS BACTERIA ASSOCIATED WITH APPLE SCAR PEEL DISEASE

作　　者：刘宏迪[1] 冯鲁昕 王祈楷[1] 张金岐[2] 张良皖 

机构地区：[1]中国科学院微生物研究所 [2]河北省农林科学院昌黎果树研究所

出　　处：《微生物学报》1990年第6期455-463,共9页Acta Microbiologica Sinica

基　　金：国家自然科学基金

摘　　要：用苹果诱果病难养菌(Fastidious bacteria)制备抗血清包被富含A蛋白的金黄色葡萄球菌。包被菌与苹果锈果病难养菌进行共凝集试验(SA-test),产生沉淀即谓协同共凝集反应(Co-agglutination)。用此法检测了不同来源、不同品系及不同树龄的苹果、巴梨、鸭梨、二十世纪梨。对难养菌接种的实验树也进行了检测。病树样品表现明显的协同共凝集反应,健树样品则无此反直。检测难养菌接种的实验树也证实了回接的成功。讨论了该方法作为苹果锈果病早期快速简便诊断的可靠性,以及应用于生产实际中的可行性。SA—test was applied successfully in dete-ction of fastidious bacteria associated withapple scar peel disease in apple and pear ofvarious cultivars with or without symptoms.The affected apple saplings which had beeninfected by grafting or inoculated by infusionwith a suspension of the bacteria in questioncould also be detected successfully whetherthe symptoms appeared or not.The usefulness of this test as a rapiddiagnosis method in the orchard was discus-sed.

关 键 词：苹果 锈果病 难养菌 金葡萄 

分 类 号：S434.611.1[农业科学—农业昆虫与害虫防治]