莪术油通过诱导细胞凋亡抑制人乳腺癌MDA-MB-231细胞增殖  

Ezhu oil inhibits human breast cancer cell line MDA-MB-231 proliferation by inducing apoptosis

作　　者：李小华[1] 孙毅毅[2] 

机构地区：[1]遂宁市第一人民医院药剂科,四川遂宁629000 [2]成都医学院,四川成都610083

出　　处：《西部医学》2011年第5期811-813,共3页Medical Journal of West China

摘　　要：目的探讨莪术油对乳腺癌细胞株MDA-MB-231增殖的抑制作用。方法水蒸汽蒸馏法提取莪术油。不同浓度的莪术油处理MDA-MB-231细胞,MTT检测干预后细胞生长抑制率,LDH试剂盒检测LDH释放,Hoechst33342荧光染色测定细胞凋亡,Western Blotting测定抗凋亡蛋白Bcl-2、促凋亡蛋白Bax的表达。结果莪术油可剂量依赖性的抑制MDA-MB-231细胞的增殖;增加细胞内LDH在细胞培养基的释放。Hoechst 33342染色显示莪术油诱导细胞凋亡,Western Blotting显示莪术油处理降低Bcl-2蛋白表达,增加Bax蛋白表达。结论莪术油通过调节Bcl-2、Bax蛋白的表达诱导细胞株凋亡抑制乳腺癌MDA-MB-231细胞株的增殖。Objective To study the anti-proliferative effect of Ezhu oil in human breast cancer line MDA-MB-231 cells.Methods The Ezhu oil was obtained by steam distillation.MDA-MB-231 cells were treated with different concentrations of Ezhu oil.The effects of Ezhu oil on MDA-MB-231 cells proliferation,intracellular LDH release were determined by MTT and LDH assay respectively.The apoptosis was monitored with Hoechst 33342 staining and the protein expression of Bcl-2 and Bas was determined with Western Blotting assay.Results Ezhu oil could significantly inhibite MDA-MB-231 cell proliferation and increase intracellular LDH releasing to culture medium.Hoechst 33342 staining showed that Ezhu oil induce apoptosis,decrease Bcl-2 protein expression and increase Bax protein expression.Conclusions Ezhu oil inhibits MDA-MB-231 cell proliferation by inducing apoptosis mediated by regulating Bcl-2 and Bax protein expression.

关 键 词：莪术油 乳腺癌 凋亡 细胞增殖 

分 类 号：R737.9[医药卫生—肿瘤]