莱菔硫烷抑制鼻咽癌细胞CNE-2中MMP-9的活性和NF-κB的激活  

Sulforaphane inhibits matrix metalloproteinase-9 enzymatic activity and NF-κB activation in CNE-2 cells

作　　者：余盛富[1] 李谨[1] 张功秀[1] 

机构地区：[1]十堰市人民医院耳鼻喉科,湖北十堰442000

出　　处：《西部医学》2011年第5期835-837,841,共4页Medical Journal of West China

摘　　要：目的探讨莱菔硫烷（sulforaphane,SFN）对鼻咽癌细胞CNE-2细胞基质金属蛋白酶-9（matrix metallo-proteinase,MMP-9）的活性及对核转录因子κB（nuclear factorκB,NF-κB）激活的影响。方法体外培养CNE-2细胞,用SFN预处理后,四氮唑盐酶还原法检测细胞的增殖情况,以及LDH的漏出率;明胶酶谱实验和实时逆转录PCR检测MMP-9的酶活性和mRNA表达水平;Western blot检测NF-κB p65亚基的转位情况。结果 0、20、40、50μMSFN处理24~72h后,均能抑制CNE-2的生长,LHD漏出率实验显示SFN对细胞无明显的毒性;SFN能明显抑制MMP-9的活性和mRNA的表达水平,同时能抑制细胞核内p65的水平。结论 SFN对CNE-2细胞生长以及MMP-9的活性具有抑制作用,它可能通过抑制NF-κB激活,从而发挥抗肿瘤作用。Objective This study was designated to investigate the effects of sulforaphane on the matrix metalloproteinase（MMP）-9 enzymatic activity and nuclear factor κB activation in a nasopharyngeal carcinoma cell line,CNE-2.Methods Cultured CNE-2 cells were incubated with sulforaphane.Microculture tetrazolium test（MTT assay）,Lactate dehydrogenase（LDH） release,Gelatin zymography,Western blot and reverse transcription PCR were employed to appraise the effect of sulforaphane on cell proliferation,cytotoxicity,metastatic potential,NF-κB activation,and MMP-9 mRNA expression in CNE-2 cells.Results 0,20,40 and 50μM of SFN inhibited cell proliferation,MMP-9 enzymatic activity,p65 translocation in a dose-dependent manner without exerting any cytotoxicity effect.In addition,an expressive decrease in mRNA levels of MMP-9 was observed.Conclusion Sulforaphane can inhibit cell proliferation and invasive potential of CNE-2 cells,and it can be through inhibition of NF-κB activation.

关 键 词：莱菔硫烷 肝细胞癌 基质金属蛋白酶-9 

分 类 号：R739.6[医药卫生—肿瘤]