新木脂素VB-1对人卵巢癌CoC1细胞生长的影响  

Effects of Neolignan VB-1 on the Growth of Human Ovarian Cancer CoC1 Cells

作　　者：曾凡香[1] 唐爱琼[1] 刘杰[1] 谢宛玉[1] 

机构地区：[1]南华大学第一附属医院妇产科,湖南衡阳421001

出　　处：《中南医学科学杂志》2011年第1期33-36,共4页Medical Science Journal of Central South China

摘　　要：目的研究新木脂素(VB-1)对人卵巢癌CoC1细胞生长的影响。方法 MTT还原法测定细胞活性;琼脂培养集落形成法检测细胞集落形成能力;碘化丙啶(PI)染色、流式细胞术分析细胞周期;Western Blotting法分析CDK2、CDK4和p21WAF1/CIP1蛋白表达。结果 VB-1显著抑制CoC1细胞活性(P<0.01),呈浓度依赖性,VB-1以浓度依赖方式抑制CoC1细胞集落形成能力(P<0.01),VB-1阻滞CoC1细胞周期于G1期(P<0.05)。VB-1下调CDK2和CDK4蛋白表达(P<0.05),同时上调p21WAF1/CIP1蛋白表达P<0.05)。结论 VB-1具有抑制CoC1细胞生长作用,其作用机制可能与阻滞细胞周期进展相关。Objective To investigate whether（VB-1）,a neolignan,inhibits the growth of human ovarian cancer CoC1 cells.Methods Cell viability was examined using MTT assay.Colony formation in CoC1 cells was detected using colony formation assay in soft agar.Cell cycle was analyzed by flow cytometry（FCM） using PI fluorescence staining.The expression of CDK2,CDK4 and p21WAF1/CIP1 protein was analyzed by Western Blot.Results VB-1 significantly inhibited the cell viability in CoC1 cells（P0.01）,in a concentration-dependent manner.VB-1 reduced the colony formation in CoC1 cells（P0.05）.FCM analysis after PI stainning indicated that the percentage of G1 cell population in CoC1 cells was increased by treatment with VB-1（P0.05）.VB-1 obviously decreased the expression of CDK2 and CDK4 protein（P0.05）,and upregulated the expression of p21WAF1/CIP1 protein（P0.05）.Conclusion VB-1 has the inhibitory effects on growth of human ovarian cancer CoC1 cell line,and its mechanisms are involved in arresting the progression of cell cycle.

关 键 词：卵巢癌 木脂素 CDK2 CDK4 P21WAF1/CIP1 

分 类 号：R737.31[医药卫生—肿瘤]