Comparison of methods of purification and identification of Verticillium dahliae toxins  

作　　者：JIA Zhi-qi SHI Fu-mei YUAN Hai-yong HOU Yan-yan LI Ying-zhang 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China

出　　处：《Journal of Life Sciences》2007年第1期67-72,81,共7页生命科学（英文版）

基　　金：Acknowledgments： This research was supported by National Natural Science Foundation of China （No. 3017555）, （No. 30170087）.

摘　　要：The complexes excreted by VerticiUium dahliae are phytotoxins, which are responsible for most of the symptoms associated with Verticillium wilt disease. Verticillium dahliae toxins （VD-toxins） can be purified by different methods. In the present study, we reported a simpler, more effective method to purify VD-toxins. The supematant of V. dahliae culture was frozen, lyophilized and dialyzed by 1 kDa Dialysis Membranes （MWCO）. We also partially identified the characteristics of the purified VD-toxins. The results showed that the components of VD-toxins include glycoprotein within 35.8-83.2 kDa. The phytotoxic activity of VD-toxins was remained after VD-toxins were pretreated by high temperature, Concanavalin-A, and proteinase E, respectively. These data suggest that VD-toxins are heat-stable, and the protein fraction and glycosyl are both important contributors to the phytotoxic activity. VD-toxins purified effectively from the culture filtrates of V. dahliae may help in further understanding the mechanisms of interactions between V. dahliae and plants.

关 键 词：GLYCOPROTEIN PHYTOTOXICITY PURIFICATION Verticillium dahliae toxins 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治] X503.23[农业科学—植物保护]