MTHFR基因多态性对1,3-丁二烯作业工人DNA损伤的影响  

Effects of MTHFR polymorphisms on DNA damage among workers exposed to 1,3-butadiene

作　　者：刘楠[1] 王学生[1] 崔涛[2] 孟会林[2] 程娟[2] 肖经纬[2] 李斌[2] 

机构地区：[1]河北联合大学公共卫生学院河北省煤矿卫生与安全实验室,河北唐山063000 [2]中国疾病预防控制中心职业卫生与中毒控制所

出　　处：《毒理学杂志》2011年第1期13-16,共4页Journal of Toxicology

基　　金：国家自然科学基金(30471471)

摘　　要：目的探讨亚甲基四氢叶酸还原酶(MTHFR)基因多态性与1,3-丁二烯作业工人DNA损伤易感性的关系。方法选取255名1,3-丁二烯作业工人和60名行政人员作为研究对象,采用碱性彗星试验评价个体外周血淋巴细胞DNA损伤,聚合酶链反应-限制性片段长度多态性方法分析MTHFR基因多态性。结果暴露组中MTHFR1298位点AA基因型个体彗星尾距(4.7)显著高于AC(4.5)或AC+CC(4.4)基因型个体。而MTHFR基因单体型分析则未发现有显著性差异。结论 1,3-丁二烯作业工人MTHFR A1298C位点的多态性可能影响其染色体损伤的易感性。Objective To investigate the association between MTHFR polymorphisms and DNA damage in 1,3-butadiene（BD）-exposed workers.Methods 255 BD-exposed workers and 60 controls were recruited in this study.DNA damage in peripheral blood lymphocytes was measured by alkaline comet assay.And the polymorphisms of MTHFR were detected by PCR-RFLP.Results Multivariate analysis of covariance revealed that in BD-exposed workers,AA genotype of A1298C exhibited higher Olive TM（4.7） than that of AC（4.5） or AC ＋ CC genotypes（4.4） significantly.The frequencies of Olive TM of 677T-1298A/677T-1298C haplotype pair were not significantly different.Conclusions The genetic polymorphisms of MTHFR A1298C could influence the chromosomal damage levels in BD-exposed workers.

关 键 词：MTHFR基因 基因多态性 单体型 彗星试验 

分 类 号：R114[医药卫生—卫生毒理学]