RAR-β_2基因启动子异常甲基化与散发性乳腺癌的关系  

Methylation of Retinoic Acid Receptor β_2 Promoter in Sporadic Breast Cancer Tissue

作　　者：谢飞[1] 向希映 张吉才[1] 陶建蜀[3] 冯景[1] 

机构地区：[1]湖北医药学院附属太和医院检验部,湖北十堰442000 [2]湖北省宜昌市中心医院检验科 [3]贵阳医学院附院临床生化科

出　　处：《肿瘤防治研究》2011年第4期408-410,415,共4页Cancer Research on Prevention and Treatment

基　　金：湖北省自然科学基金资助项目(2008CDB195)

摘　　要：目的探讨RAR-β2基因(retinoic acid receptor β2 gene)在散发性乳腺癌中启动子异常甲基化状况,进而探讨在散发性乳腺癌早期诊断中的检测价值。方法用甲基化特异性PCR(MSP)法和实时荧光定量PCR法对62例散发性乳腺癌病人的癌组织,15例增生的乳腺组织,10例正常乳腺组织进行RAR-β2基因的异常甲基化的检测。结果散发性乳腺癌癌组织中50%(31/62)发生了RAR-β2基因甲基化,增生的乳腺组织标本中20%(3/15)发生了RAR-β2基因启动子甲基化,正常的乳腺组织中均未检测到RAR-β2基因的甲基化。散发性乳腺癌癌组织中甲基化率与年龄、绝经与否无相关性,与淋巴结转移和临床分期有相关性(P<0.05);散发性乳腺癌与增生的乳腺组织和正常的乳腺组织间的差异有统计学意义(P<0.05)。结论 RAR基因甲基化可能参与了乳腺癌的发生发展过程,与乳腺癌的病理分期和预后评估等方面有关。Objective To investigate the methylation status of RAR-β2 gene and its dignostical value in sporadic breast cancer.Methods Sixty-two sporadic breast cancer tissues,15 benign breast tissues and 10 normal breast tissues were collected and subjected to methylation-specific PCR（MSP） and real-time quantitive PCR.Results Methylated rates of RAR-β2 gene promoter were observed in 50%（31/62） of breast cancer tissues,and in 20%（3/15） in benign proliferative breast tissues,but not in normal breast tissues.The prevalence of RAR-β2 gene promoter methylation in malignant was significantly higher than those in benign and normal breast tissues.No association was observed between RAR-β2 gene promoter methylation and patient＇s clinical-biologic characteristics including age,menostasis,but association was observed between RAR-β2 gene promoter methylation and the patients of the lymph node metastasis and clinical typing.Conclusion Our results suggest that promoter hypermethylation of RAR-β2 gene may be involved in the carcinogenesis of sporadic breast cancer.There is the correlation between promoter hypermethylation of RAR-β2 gene and the patients of the lymph node metastasis and clinical typing.

关 键 词：散发性乳腺癌 DNA甲基化 RAR-β2基因 甲基化特异性PCR 

分 类 号：R737.9[医药卫生—肿瘤]