纤维粘连蛋白EDA片段调控结肠癌SW480细胞的增殖、凋亡  

Fibronectin EDA domain regulates proliferation and apoptosis of colorectal cancer SW480 cells

作　　者：耿培亮[1] 欧娟娟[1] 萨日娜[1] 何金霞[1] 谢赣丰[1] 刘平[1] 梁后杰[1] 

机构地区：[1]第三军医大学西南医院肿瘤科,重庆400038

出　　处：《第三军医大学学报》2011年第9期920-923,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(30973430)~~

摘　　要：目的研究纤维粘连蛋白EDA片段对结肠癌SW480细胞增殖、凋亡的影响。方法慢病毒感染结肠癌SW480细胞干扰纤维粘连蛋白EDA片段表达,建立纤维粘连蛋白EDA片段低表达的SW480细胞。利用MTT检测干扰前后细胞增殖的变化,并采用流式细胞术检测干扰前后细胞凋亡的变化。同时使用Western blot分别检测干扰前后凋亡相关蛋白Bax和Bcl-2的表达变化。结果慢病毒感染结肠癌SW480细胞后,纤维粘连蛋白EDA片段持续低表达。MTT法结果显示,干扰纤维粘连蛋白EDA片段24、48、72、96 h后细胞生长速度明显降低。转染组与未转染组及空载体组比较,差异均有统计学意义(P<0.05)。流式细胞术显示未转染组、空载体组及转染组的细胞凋亡率分别为(3.09±1.13)%、(3.71±0.42)%、(47.76±1.51)%,差异有统计学意义(P<0.05)。Western blot的结果显示干扰纤维粘连蛋白EDA片段后促凋亡因子Bax表达上调,同时,凋亡抑制因子Bcl-2表达降低。结论纤维粘连蛋白EDA片段对结肠癌SW480细胞具有促增殖、抗凋亡作用。Objective To determine the effect of fibronectin extra type Ⅲ domain A（EDA-FN） on the proliferation and apoptosis of human colorectal cell line SW480.Methods The expression of EDA-FN in SW480 cells was silenced by lentiviral infection.Expression of EDA-FN was examined by Western blot analysis.MTT assay was used to analyze cell viability after infection.Cell apoptosis was analyzed by flow cytometry.The expression of Bax and Bcl-2 were examined by Western blot analysis.Results As compared with the blank control cells（without lentiviral infection） and negative control cells（empty vector infection）,the infection of lentiviral inhibited the expression of EDA-FN in SW480 cells.The proliferative capacity of cells was decreased in 24,48,72 and 96 h after lentiviral infection,and all were significantly lower than blank control group and negative control group（P0.05）.The apoptotic rate of SW480 cells after lentiviral infection was（47.76±1.51）%,significantly higher than those of blank control group and negative control group [（3.09±1.13）% and（3.71±0.42）% respectively,P0.05].Western blot analysis showed that the expression of Bax was increased,while that of Bcl-2 was decreased.Conclusion EDA-FN induces the proliferation and inhibits the apoptosis of human colorectal cacer SW480 cells.The inhibitor of EDA-FN may be a new target in the treatment of the malignant tumors.

关 键 词：纤维粘连蛋白 RNAI 结肠癌 增殖 凋亡 

分 类 号：R394.3[医药卫生—医学遗传学] R735.35[医药卫生—基础医学]