D-环丝氨酸促进大鼠条件性恐惧消退记忆的长期保持  

Promotion effect of D-cycloserine on long-term retention of rat extinction memory of conditioned fear

作　　者：张钰[1] 李敏[1] 

机构地区：[1]第三军医大学护理系心理学教研室,重庆400038

出　　处：《第三军医大学学报》2011年第9期951-953,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30670712);重庆市自然科学基金(CSTC2009BB5014)~~

摘　　要：目的研究D-环丝氨酸(D-serine,DCS)对条件性恐惧消退记忆的长时保持的影响。方法成年雄性SD大鼠40只,采用完全随机方法分为空白对照组、消退对照组、消退训练组、消退训练前DCS干预组、消退训练后DCS干预组,每组8只。在消退训练后3 d时进行僵立行为测试与旷场行为测试。结果①消退训练组的僵立时间百分比[(39.77±22.69)%]显著低于消退对照组[(61.46±11.10)%](P<0.05),但仍高于空白对照组[(2.82±1.27)%](P<0.01);旷场测试中消退训练组的中央路程百分比及直立次数[(2.82±2.89)%,(7±4)]显著高于消退对照组[(0.50±0.84)%,(3±4)],但低于空白对照组[(19.29±6.08)%,(20±6)](P<0.05);②消退训练后DCS干预组的僵立时间百分比与旷场行为测试成绩与其他组比较均有统计学差异(P<0.05)。结论消退训练后给予D-环丝氨酸能够促进条件化恐惧消退记忆的长时保持。Objective To investigate the influence of D-cycloserine（DCS） on the long-term retention of rat extinction memory of conditioned fear.Methods Forty healthy male adult SD rats were randomly divided into five groups（n=8）： a blank control group,an extinction control group,an extinction training group,a group with DCS treatment before extinction training,and a group with DCS treatment after extinction training.Following fear conditioning,which includes auditory conditioned stimulus（CS） paired with foot-shock unconditioned stimulus（US）,rats received non-reinforced exposure to CS for extinction training.CS-elicited freezing test for fear assessment and open-field test were conducted 3 d after extinction training.Results The percentage of freezing time of the extinction training group[（39.77±22.69）%] was significantly lower than that of the extinction control group[（61.46±11.10）%]（P0.05）,but significantly higher than that of the blank control group[（2.82±1.27）%]（P0.01）.The percentage of central distance and standing frequency of the extinction training group [（2.82±2.89）% and 7±4] in the open-field test were both significantly higher than those of the extinction control group [（0.50±0.84）% and 3±4],but lower than those of the blank control group [（19.29±6.08）% and 20±6]（P0.05）.The percentage of freezing time and open-field test score of the group with DCS treatment after extinction training were significantly different from those of other four groups（P0.05）.Conclusion DCS administration after extinction training promotes long-term retention of extinction memory of conditioned fear.

关 键 词：条件性恐惧 恐惧消退 D-环丝氨酸 NMDA受体 

分 类 号：R749.05[医药卫生—神经病学与精神病学] R749.5[医药卫生—临床医学]