低形态学评分卵裂期胚胎的X、Y及18号染色体分析  

Analysis of Chromosome X,Y and 18 in Cleavage Stage Embryos with Low Morphological Scores by the Fluorescent in Situ Hybridization

作　　者：罗海宁[1] 杨清秀[1] 杜湧瑞[1] 鲁振宇[1] 姚汝强[1] 王毅娜[1] 张云山[1] 

机构地区：[1]天津市中心妇产科医院生殖医学中心,300052

出　　处：《天津医药》2011年第4期292-294,386,共4页Tianjin Medical Journal

基　　金：天津市科技发展计划项目(项目编号:05YFGZGX0900)

摘　　要：目的:分析低形态学评分的D3卵裂期胚胎X、Y及18号染色体的非整倍体情况。方法:收集体外受精-胚胎移植术后剩余的废弃胚胎,胚胎固定后采用荧光原位杂交技术检测细胞核内的X、Y及18号染色体。结果:共收集废弃胚胎60个,其中正常胚胎29个,18号染色体异常胚胎12个,X/Y染色体异常胚胎8个,18、X/Y染色体均异常胚胎11个。观测到荧光信号的细胞核有233个,其中正常细胞核115个(49.4%),异常细胞核118个(50.6%)。18号染色体非整倍体率40.8%,X/Y染色体非整倍体率33.5%,差异无统计学意义(χ2=2.65,P>0.05)。18号染色体三体发生率(21.9%)与X/Y染色体三体发生率(12.0%)差异有统计学意义(χ2=11.58,P<0.01)。结论:D3形态评分低的胚胎有相当大的比例存在染色体异常,通用的胚胎形态学评分系统能够在一定程度上反映胚胎的染色体情况。Objective：To evaluate the aneuploidy status of chromosome X,Y and 18 in day 3 cleavage stage embryos with low morphological scores by the fluorescent in situ hybridization（FISH）.Methods：The discarded embryos from in vitro fertilization-embryo transfer（IVF-ET） cycles were collected for this study.The nuclei of all the blastomeres were fixed,in which the chromosome X,Y and 18 were analyzed by FISH.Results：Fluorescent signals were successfully detected in 233 nuclei,in which 115（49.4%） nuclei were normal euploidy of chromosome X,Y and 18,while 118（50.6%） were abnormal.The aneuploidy rate of chromosome 18 and X/Y were 40.8% and 33.5% respectively,but there no significant difference（χ2 =2.65,P 0.05）.The trisome rate of chromosome 18 was significantly higher than that of chromosome X/Y（21.9% vs12.0%,χ2=11.58,P 0.01）.Conclusion：A majority of the day 3 cleavage stage embryos with low morphological scores was proved to be chromosomal abnormal.The general embryo morphology scoring criteria could predict the embryo euploidy status to some extent.

关 键 词：胚胎发育 染色体 人 X 染色体 人 Y 染色体 人 18对 染色体畸变 原位杂交 荧光 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]