叔丁基对苯二酚对百草枯神经细胞毒性和氧化应激的拮抗作用  

Antagonism of tert-butylhydroquinone on neurotoxicity and oxidative stress induced by paraquat in PC12 cells

作　　者：李煌元[1] 吴思英 林炜[1] 王章敬[1] 叶鹏凌[1] 韩秋凤[3] 

机构地区：[1]福建医科大学公共卫生学院,福建省重点建设大学环境与健康重点学科,环境与健康研究所毒理学与化学物安全性评价研究室,职业与环境卫生学系,福州350004 [2]公共卫生学院流行病与卫生统计学系 [3]福建医科大学公共卫生学院流行病与卫生统计学系,福州350004

出　　处：《中华劳动卫生职业病杂志》2011年第4期270-274,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（30800936）

摘　　要：目的 研究叔丁基对苯二酚（tBHQ）预处理对百草枯（PQ）致大鼠肾上腺嗜铬细胞瘤（PC12）细胞毒性和氧化应激的拮抗作用.方法 将PC12细胞分为溶剂对照组及100、300 μmol PQ处理组.用终浓度为40 μmol/LtBHQ预处理PC12细胞4 h再分别用终浓度0、100、300 μmol/L PQ处理细胞24或48 h,用噻唑蓝（MTT）法检测细胞毒性,用Annexin V-FITC/PI法流式细胞仪（FCM）检测细胞凋亡,用硫代巴比妥酸法测定细胞丙二醛（MDA）含量.结果 用40μmol/LtBHQ预处理后再用100、300μ,mol/L PQ处理PC12细胞24 h细胞存活率分别较100、300 μmol/L PQ组增高,差异有统计学意义（P＜0.05,P＜0.01）;用40μmol/LtBHQ预处理后再用100μmol/LPQ处理PC12细胞48 h细胞存活率较100μmol/LPQ处理组细胞存活率增高,差异有统计学意义（P＜0.01）;用40μmol/LtBHQ预处理后再用100、300μmol/PQ处理PC12细胞24 h后,细胞凋亡率分别较100、300 μmol/L PQ下降,差异有统计学意义（P＜0.05,P＜0.01）;用40μmol/LtBHQ预处理后再用100、300μmol/LPQ处理PC12细胞24 h后,MDA含量分别较100、300 μmol/L PQ组下降,MDA含量分别为相应对照组的0.61、0.57倍,差异有统计学意义（P＜0.05）.结论 tBHQ预处理可削弱PQ致PC12细胞的细胞毒性、细胞凋亡以及氧化应激作用.Objective To investigate the protective effects of the tert-butylhydroquinone （tBHQ）pretreatment on neurotoxicity and oxidative stress induced by paraquat （PQ） in PC12 cells. Methods Cytoyoxicity of PC12 cells was measured by MTT assay, following the PC12 cells treatment with different concentrations of 100, 300 μmol/L PQ for 24 h and 48 h. PC12 cells were pretreated with or without 40 μmol/L tBHQ for 4 h, PC 12 cells were exposed to PQ at the doses of 0, 100, 300 μmol/L for 24 h and 48 h, respectively.The viability of PC 12 cells was measured by MTT assay, the apoptosis rates of PC 12 cells were detected by flow cytometry （FCM） and the malondialdehyde （MDA） levels of PC 12 cells were examine by thiobarbituric acid （TBA） method. Results When the exposure doses of PQ were 100 and 300 μmol/L for 24 h, the viability of PC 12 cells pretreated with tBHQ was significantly higher than that of PC 12 cells only exposed to PQ （P＜0.05 or P＜0.01）. When the exposure dose of PQ was 100μmol/L for 48 h, the viability of PC12 cells pretreated with tBHQ was significantly higher than that of PC12 cells only exposed to PQ （P＜0.01）. When the exposure doses of PQ were 100 and 300 μmol/L for 24 h, the apoptosis rates and MDA levels of PC12 cells pretreated with tBHQ were significantly lower than those of PC12 cells only exposed to PQ （P＜0.05 or P＜0.01）. Conclusions tBHQ preteatment can reduce the cytotoxicity, apoptosis and oxidative stress induced by PQ in PC12 cells.

关 键 词：百草枯 叔丁基对苯二酚 神经元 丙二醛 

分 类 号：R[医药卫生]