VEGF siRNA增强白血病HL60细胞对阿糖胞苷药物的敏感性  

VEGF siRNA enhances sensitivity of leukemia cells to Ara-C

作　　者：荆春霞[1] 张洹[2] 杨光[3] 

机构地区：[1]暨南大学医学院流行病学教研室,广东广州510632 [2]暨南大学医学院血液病研究所,广东广州510632 [3]暨南大学医学院人体寄生虫学系,广东广州510632

出　　处：《基础医学与临床》2011年第5期546-550,共5页Basic and Clinical Medicine

基　　金：广东省科技重点基金(2001-Z-037-01);广东省自然科学基金(021195)

摘　　要：目的探讨VEGF siRNA是否能增强白血病细胞HL60对化疗药物阿糖胞苷的敏感性。方法体外转录合成VEGF siRNA,转染HL60细胞,采用CCK8法检测细胞增殖的情况,RT-PCR方法检测细胞血管内皮细胞生长因子(VEGF)mRNA的表达水平,ELISA方法定量检测细胞培养液VEGF蛋白的表达水平,用流式细胞仪检测细胞的凋亡。结果 VEGFsiRNA可以提高HL60细胞对阿糖胞苷的敏感性,VEGFsiRNA与阿糖胞苷联用能显著抑制HL60细胞的存活,抑制率为82.9%(F=121.63,P<0.01)。VEGF siRNA能降低VEGF mRNA121(F=17.17,P<0.01)和VEGF蛋白(F=3290.49,P<0.01)的表达水平。但是VEGF siRNA没有促进阿糖胞苷诱导的细胞凋亡水平。结论 VEGF siRNA通过抑制细胞的增殖来增强HL60细胞对阿糖胞苷的敏感性。Objective To explore the effects of VEGF siRNA on chemosensitivity of leukemia cells to Ara-C.Methods VEGF siRNA was constructed and then transferred to HL60 cells.The proliferation was detected by CCK8 method.The level of VEGF mRNA was detected by RT-PCR.The expression level of VEGF protein was determined by ELISA.The cell apoptosis was detected by flow cytometry.Results VEGF siRNA increased the sensitivity of leukemia cells to Ara-C,VEGF siRNA plus Ara-C significantly decresed the survival of HL60 cells in which the inhibition rate was 82.9 percentages（F=121.63,P0.01）.VEGF siRNA decreased significantly expression of VEGF mRNA121（F=17.17,P0.01） and VEGF protein（F=3290.49,P0.01）.However,VEGF siRNA didn＇t promote the level of apoptosis induced by Ara-C.Conclusion VEGF siRNA increases the sensitivity of leukemia cells to Ara-C by inhibiting proliferation.

关 键 词：血管内皮生长因子 SIRNA 阿糖胞苷 药物敏感性 

分 类 号：R733.7[医药卫生—肿瘤]