大肠腺瘤与大肠腺癌肿瘤相关基因的差异表达  

Differential expression of tumor-related genes between colorectal adenoma and adenocarcinoma

作　　者：曾伟[1] 南清振[1,2] 戴益琛[1] 朱小三[1,3] 陈章兴[1] 谢军培[1] 傅育卡[1] 林园园[1] 

机构地区：[1]中国人民解放军174医院消化内科,福建省厦门市361003 [2]广州市南方医院消化科,广东省广州市510515 [3]南昌大学研究生院医学部,江西省南昌市330006

出　　处：《世界华人消化杂志》2011年第8期868-873,共6页World Chinese Journal of Digestology

基　　金：2009年度军区医学科技创新课题基金资助项目;No.09MA066;厦门市科技计划医疗卫生创新基金资助项目;No.3502Z20084031~~

摘　　要：目的:探索大肠腺瘤与大肠腺癌差异表达的肿瘤相关基因.方法:利用基因芯片技术筛选大肠腺瘤与大肠腺癌差异表达的肿瘤相关基因,比较两组基因特点,寻找大肠腺癌重要致病基因,并以RT-PCR对部分差异表达基因进行检测来验证芯片结果.结果:(1)大肠腺瘤差异表达的肿瘤相关基因9个,均上调;(2)大肠腺癌差异表达的肿瘤相关基因47个,其中上调29个,下调18个;(3)大肠腺瘤与大肠腺癌共同差异表达的肿瘤相关基因有17个,其中14个基因均上调;(4)CAPN1、JUNB、ELF3、IER3等基因在大肠腺癌与大肠腺瘤中差异表达上调,但在大肠腺癌中显著表达,PDGFRA及PLAGL1基因在大肠腺瘤中表达上调,而在大肠腺癌相反.结论:大肠腺瘤差异表达的肿瘤相关基因较少,大肠腺癌则明显增多;CAPN1、JUNB、ELF3、IER3、PDGFRA及PLAGL1等基因可能为大肠腺癌重要致病基因.AIM： To identify differentially expressed tumorrelated genes between colorectal adenoma and adenocarcinoma.METHODS： Gene chip technology was used to screen differentially expressed tumorrelatedgenes between colorectal adenoma and adeno- carcinoma.RT-PCR was then performed to validate microarray results.RESULTS： Nine differentially expressed tumorrelated genes were expressed only in colorectal adenoma,all of which were up-regulated.Forty- seven differentially expressed tumor-related genes were expressed only in adenocarcinoma,of which 29 were up-regulated and 18 down- regulated.Seventeen differentially expressed tumor-related genes were expressed in both colorectal adenoma and adenocarcinoma,of which 14 were up-regulated.CAPN1,JUNB,ELF3 and IER3 genes were up-regulated in both colorectal adenoma and adenocarcinoma,but the up-regulation was more signifi cant in colorectal adenocarcinoma.PDGFRA and PLAGL1 genes were up-regulated in colorectal adenoma but down-regulated in adenocarcinoma.CONCLUSION： Colorectal adenoma expresses less differentially expressed tumor-related genes than adenocarcinoma.CAPN1,JUNB,ELF3,IER3,PDGFRA and PLAGL1 genes may be involved in the pathogenesis of colorectal adenocarcinoma.

关 键 词：大肠腺瘤 基因芯片技术 大肠腺癌 

分 类 号：R735.34[医药卫生—肿瘤]