Effects of contact guidance and gravity on L929 cell orientation  

作　　者：ZHOU Feng YUAN Lin MEI Yan CHEN Hong 

机构地区：[1]School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, China [2]College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China

出　　处：《Chinese Science Bulletin》2011年第10期977-981,共5页

基　　金：supported by the National Natural Science Foundation of China(20634030);the Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Education(20071108)

摘　　要：Contact guidance and external force field are two important factors that influence cell orientation.Microgroove-like patterns on PDMS substrates were fabricated using soft-lithography,and the effects of gravitational field direction relative to the pattern,on L929 cell orientation were investigated.The majority of cells were aligned along the grooves when gravity was parallel to the groove direction,because of contact guidance.When gravity was perpendicular to the groove direction,cell alignment decreased noticeably.Although contact guidance induced by the pattern played a dominant role in determining cell orientation,gravity also had an important influence when the field direction was perpendicular to the groove direction.No synergistic influence on cell alignment was observed when the field direction was parallel to the groove direction.Contact guidance and external force field are two important factors that influence cell orientation. Microgroove-like patterns on PDMS substrates were fabricated using soft-lithography, and the effects of gravitational field direction relative to the pattern, on L929 cell orientation were investigated. The majority of cells were aligned along the grooves when gravity was parallel to the groove direction, because of contact guidance. When gravity was perpendicular to the groove direction, cell alignment decreased noticeably. Although contact guidance induced by the pattern played a dominant role in determining cell orientation, gravity also had an important influence when the field direction was perpendicular to the groove direction. No synergistic influence on cell alignment was observed when the field direction was parallel to the groove direction.

关 键 词：L929细胞 重力 磁场方向 PDMS 光刻技术 单元格 凹槽 外力场 

分 类 号：Q814.2[生物学—生物工程]