人脑干胶质瘤N—cadherin、E—cadherin及β-catenin的表达及其意义  

N -cadherin, E -cadherin and β- catenin expressions in human brainstem gliomas

作　　者：马俊艳[1] 武文浩[2] 田永吉[2] 历俊华[1] 李德志[2] 郝淑煜[2] 阴鲁鑫[2] 张力伟[2] 万虹[1] 

机构地区：[1]首都医科大学北京市神经外科研究所,100050 [2]首都医科大学附属北京天坛医院神经外科

出　　处：《中华神经外科杂志》2011年第4期518-522,共5页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金（30772237,30900479）

摘　　要：目的 探讨神经-钙粘素（N-cadherin）、上皮-钙粘素（E-cadherin）及相关因子β-连环蛋白（β-catenin）在高、低级别人脑干胶质瘤中的表达及其意义.方法 采用免疫组化方法和RT-PCR法检测不同级别脑干胶质瘤N-cadherin、E-cadherin及β-catenin的表达.结果 N-cadherin蛋白在高、低级别脑干胶质瘤中表达差异无统计学意义（P＞0.05）,mRNA的表达在脑干胶质瘤中随着肿瘤级别的升高而增高,高、低级别间差异有统计学意义（t=2.711,P＜0.05）;E-cadherin蛋白和mRNA在高、低级别脑干胶质瘤中均表达缺失或减少,组间差异无统计学意义（P＞0.05）;β-catenin蛋白的表达在脑干胶质瘤中随着肿瘤级别的升高而增高,高、低级别间差异有统计学意义（P＜0.05）,mRNA的表达在脑干各级别胶质瘤中差异无统计学意义（P＞0.05）.结论 N-cadherin转录水平的过度表达及β-catenin蛋白水平的过度表达可能与人脑干胶质瘤的恶性进展有关,而E-cadherin转录和蛋白水平的表达下调可能与此无关.Objective To explore the expression and significance of N - cadherin, E - cadherin and β - catenin in human brainstem gliomas. Methods N - cadherin, E - cadherin and β - catenin proteins and mRNA were detected in 38 cases of brainstem gliomas using immunohistochemistry and RT - PCR Methods. Results There was no significant difference in the expression of N - cadherin between grades Ⅰ ～ Ⅱ and grade Ⅲ ～ Ⅳ （ P 〉 0. 05） . The expression of N - cadherin mRNA in human brainstem gliomas of grade Ⅰ ～ Ⅱ was significantly lower than that in grade Ⅲ～ Ⅳ （ t = 2. 711, P 〈 0. 05 ）. There was no significant difference in the expression of E - cadherin and E - cadherin mRNA between grade Ⅰ ～ Ⅱ and grade Ⅲ～ Ⅳ （ P 〉 0. 05 ）. The expression of β - catenin in human brainstem gliomas of grade Ⅰ ～ Ⅱ was significantly lower than that in grade Ⅲ ～ Ⅳ （ P 〈 0. 05 ）. As regard to β -catenin mRNA, there was no significant difference between grade Ⅰ ～ Ⅱ and grade Ⅲ ～ Ⅳ （ P 〉 0. 05 ）. Conclusions The over -expressionof N - cadherin and β - catenin may play an important role in the invasion and malignant progression of human brainstem gliomas.

关 键 词：脑干 神经胶质瘤 神经-钙粘素 上皮-钙粘素 Β-连环蛋白 

分 类 号：R739.4[医药卫生—肿瘤]