Rho激酶抑制剂对大鼠脑缺血损伤的脑保护作用及S100B蛋白表达的影响  

Protective effects of Rho kinase inhibitor and influence on expression of S100B protein after cerebral ischemia in rats

作　　者：刘康[1] 丁素菊[2] 吴涛[2] 仇一青[2] 郭鹏[2] 

机构地区：[1]中国人民解放军第456医院神经内科,济南250031 [2]第二军医大学长海医院神经内科

出　　处：《临床神经病学杂志》2011年第2期111-113,共3页Journal of Clinical Neurology

摘　　要：目的探讨Rho激酶抑制剂（法舒地尔）对大鼠脑缺血损伤的脑保护作用及S100B蛋白表达的影响。方法 90只雄性SD大鼠随机分为假手术组、脑缺血组和法舒地尔组。采用大脑中动脉线栓法制作大鼠局灶性脑缺血模型。术后对3组大鼠进行神经功能缺损评分（NSS）;用分光光度法测定髓过氧化物酶（MPO）活性;用干湿重法测定脑含水量;伊文思蓝（EB）法测定血-脑屏障的通透性;实时荧光定量逆转录PCR法检测缺血脑组织中S100B蛋白的表达。结果与假手术组比较,脑缺血组和法舒地尔组大鼠NSS和MPO活性明显增高,脑含水量、EB含量及S100B蛋白表达明显增加（P〈0.05~0.01）。与脑缺血组比较,法舒地尔组大鼠NSS和MPO活性明显降低,脑含水量、EB含量及S100B蛋白表达明显减少（均P〈0.05）。结论法舒地尔可通过抑制脑缺血损伤后的炎症反应发挥脑保护作用,其机制可能与下调S100B蛋白的表达有关。Objective To explore the protective effects of Rho kinase inhibitor（Fasudil） and influence on expression of S100B protein after cerebral ischemia in rats.Methods Ninety male SD rats were randomly divided into sham operation group,ischemia group and Fasudil group.Focal cerebral ischemia model rats were made by transient occlusion of middle cerebral artery.The neurological severity score（NSS） of the 3 group were detected after operation.The myeloperoxidase（MPO） activity was determined by spectrophotometer.The water content of brain was measured by dry-wet weight method.The permeability of the blood-brain barrier was evaluated by measurement of the Evans Blue（EB） content in the brain with spectrophotometer.The expression of S100B protein were detected by reverse transcription PCR.Results Compared with sham operation group,the NSS,MPO activity,brain water content,EB content and the expression of S100B were significantly increased in ischemia group and Fasudil group（P0.05-0.01）.Compared with ischemia group,the NSS,MPO activity,brain water content,EB content and the expression of S100B were significantly decreased in Fasudil group（all P0.05）.Conclusion Fasudil can make the protective effects by inhibiting inflammatory reaction of cerebral ischemia,which may be related with the decrease of the expression of S100B protein.

关 键 词：RHO激酶抑制剂 脑缺血损伤 S100B蛋白 炎症 

分 类 号：R743.3[医药卫生—神经病学与精神病学]