CD4＋CD25＋调节性T淋巴细胞在大鼠肝纤维化模型中的变化  

The changes of CD4＋ CD25＋ regulatory T lymphocyte in liver fibrosis rat model induced by carbon tetrachloride

作　　者：谷甸娜[1] 陈永平[1] 张磊[1] 张晓华[1] 卢洁[1] 郑毅[1] 郑明华[1] 

机构地区：[1]温州医学院附属第一医院感染科,325000

出　　处：《中华传染病杂志》2011年第3期139-142,共4页Chinese Journal of Infectious Diseases

基　　金：浙江省自然科学基金资助项目（Y207464）;浙江省科技厅新苗人才计划项目（2008R40G2090022）

摘　　要：目的探讨CD4＋CD25＋调节性T淋巴细胞（Tregs）在肝纤维化免疫发病机制中的作用。方法26只大鼠分为对照组（6只）和模型组（20只），通过皮下注射四氯化碳（CCl4）建立肝纤维化模型，检测血清肝功能和纤维化指标，观察肝脏病理学变化，流式细胞仪测定外周血及脾脏CD4＋CD25＋Tregs数量的变化。两组间差异比较采用t检验，相关性采用Pearson直线相关分析。结果模型组血清ALT、AST升高，Alb下降；HA、PCⅢ、ⅣC分别为（177．42±61．25）μg／L、（34．86±7．47）μg／L、（7．32±3．71）μg／L，较对照组明显升高（t=-3．670、-5．661、-3．950，均P〈0．01）；模型组肝脏组织胶原纤维大量增生，分割肝小叶并包绕成假小叶。模型组外周血中CD4＋CD25＋Tregs占CD4＋T淋巴细胞比例为（7．41±2．15）％，较对照组的（12．88±2．93）％低（t=3．752，P％0．01）；模型组脾脏中Tregs为（9．49±1．16）％，较对照组的（13．16±2．36）％亦低（t=2．793，P〈0．05）。ALT、AST与外周血和脾脏中Tregs水平呈直线负相关（ALT、AST与外周血中Tregs：r分别为-0．727、-0．698，ALT、AST与脾脏中Tregs：r=-0．663、-0．535；均P〈0．05）；而Alb与外周血和脾脏中Tregs水平无直线相关（r=0．423、0．372，均P〉0．05）；HA、PCHI、ⅣC与外周血和脾脏中Tregs水平呈直线负相关（HA、PCⅢ、ⅣC与外周血中Tregs：r=-0．719、-0．558、-0．792，HA、PCⅢ、ⅣC与脾脏中Tregs：r=-0．424、-0．685、-0．506；均P〈0．05）。结论CCl4诱导的大鼠肝纤维化模型CD4＋CD25＋Tregs的表达降低，其肝纤维化的免疫学发病机制有待进一步研究。Objective To investigate the role of CD4＋ CD25＋ regulatory T lymphocyte （Tregs） in the immunological pathogenesis of liver fibrosis. Methods Twenty-six rats were divided into two groups： control group （6 rats） and model group （twenty rats）. The rat model of liver fibrosis was induced by subcutaneous injection of carbon tetrachloride （CC14）. The serums were collected for detection of hepatic function and fibrosis parameters. Hepatic tissue samples were used to observe the histopathological changes. The flow cytometry was used to detect the proportions of Tregs in both peripheral blood and spleen. The data were evaluated by t test. The relationship between two variables was analyzed using Pearson linear correlation. Results The levels of serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were significantly increased, but the level of serum albumin （Alb） was obviously decreased. The concentrations of serum hyaluronic acid （HA）, procollagen type HI （PCⅢ）, collagen type Ⅳ （CIV） of the model group increased to （177.42±61.25） μg/L, （34.86±7.47） μg/L and （7.32±3.71） μg/L, respectively, which were higher than those in the control group （t= 3. 670, -5. 661, -3. 950, respectively; all P〈0.01）. In model group, hepatic lobules were full of collagen fibers and the hepatic pseudolobule formation was observed. The proportion of peripheral blood Tregs in CD4＋ T lymphocyte in liver fibrosis model was （7. 41 ± 2.15）%, which was significantly lower than that in control group （12.88± 2.93）% （t=3. 752, P〈 0.01）. Furthermore, the frequency of Tregs in spleen of the model group was （9. 49±1. 16）%, which was also significantly lower than that in control group （13.16 ± 2.36）% （t = 2.793, P〈0.05）. In addition, the levels of serum ALT, AST and fibrosis parameters were inversely correlated with the frequency of Tregs in spleens and peripheral blood （ALT and Tregs in blood： r=-0. 727, AST and Tregs in blood：

关 键 词：CD4阳性T淋巴细胞 白细胞介素2受体α亚单位 肝硬化 四氯化碳 疾病模型 动物 

分 类 号：R575.2[医药卫生—消化系统]