miR-34a在结直肠癌中的表达及其临床意义  

Expression of miR-34a and its clinical significance in human colorectal carcinoma

作　　者：田智丹[1] 任勇亚[2] 戚晓红[2] 冯振卿[2] 

机构地区：[1]南京医科大学附属南京第一医院病理科,南京210006 [2]南京医科大学病理学与病理生理学系,南京210029

出　　处：《临床与实验病理学杂志》2011年第4期347-350,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨miR-34a在结直肠癌中的表达及其与临床病理特征的关系。方法应用实时荧光定量PCR法对43例结直肠癌及其癌旁正常组织中miR-34a进行定量检测。结果结直肠癌组织中miR-34a表达水平明显高于癌旁正常组织,差异有统计学意义(P>0.05);miR-34a在结直肠癌组织中的表达与患者性别、年龄、肿瘤部位、肿块大小、淋巴结转移均无相关性(P>0.05),而与浸润深度、TNM分期密切相关(P<0.05)。结论 miR-34a在结直肠癌的发生、发展过程可能发挥重要作用,检测miR-34a有望成为结直肠癌新的肿瘤标记物或预后因子。Purpose To investigate the expression of miR-34a in human colorectal carcinoma and its relationship with clinicopathological features.Methods The mRNA expression of miR-34a was determined by real-time quantitative PCR in 43 colorectal carcinoma tissues and para-cancerous normal tissues.Results Real-time quantitative PCR analysis revealed that miR-34a expression in colorectal carcinoma tissues was lower than those in the paraneoplastic colorectal tissues（P0.05）.There were no significant correlations between the expression of miR-34a and gender,age,tumor location,tumor size and lymph node metastases（P0.05）,but it related to depth of invasion and TNM stage（P0.05）.Conclusions Downregulation of miR-34a expression in the colorectal carcinoma suggests that miR-34a may be involved in the carcinogenesis and development of colorectal carcinoma.miR-34a may be a novel useful tumor marker and a prognostic factor for colorectal carcinoma.

关 键 词：结直肠肿瘤 MIR-34A 实时荧光定量PCR 

分 类 号：R735.3[医药卫生—肿瘤]