APC^(Min/+)结直肠癌癌前病变小鼠模型的生物学特性  

C57BL/6J-APC^(Min/+):a mouse model of precancerosis of colorectal cancer

作　　者：叶志金[1] 郑力[1] 亓翠玲[1] 张敏[1] 唐富天[1] 王尽淘[1] 耿建国[1] 王丽京[1] 

机构地区：[1]广东药学院血管生物学研究所,广州510006

出　　处：《临床与实验病理学杂志》2011年第4期393-395,399,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(30871304);国家科技部"973"(国家重点基础研究发展计划2010CB529702)

摘　　要：目的研究C57BL/6J-APCMin/+小鼠肠道腺瘤的生物学特性。方法记录APCMin/+小鼠的繁育情况,通过统计不同周龄小鼠小肠及大肠腺瘤的数目和总体积,观察其肠道腺瘤的发生、发展规律;通过病理组织学切片,观察腺瘤的病理学变化;通过免疫组织化学染色,观察WNT信号通路相关基因的表达情况。结果 APCMin/+小鼠9周龄时肠道开始有腺瘤发生,24周龄时多数小鼠出现死亡。从9~24周龄,腺瘤体积持续增大,但小肠腺瘤数目不再增加。免疫组化染色显示腺瘤中β-catenin入核,启动WNT信号通路下游原癌基因cyclinD1活化。结论 APCMin/+小鼠是良好的结直肠癌前病变动物模型。Purpose To describe biological features of C57BL/6J-APCMin/＋ mice.Methods APCMin/＋ mice were maintained under specific pathogen free conditions.The number and size of intestinal polyps were assessed at different age point.Pathological changes of polyps were confirmed by HE staining.The gene expression in WNT signaling pathways was determined by immunohistochemistry.Results Polyps emerged at approximately 6 weeks age and most mice died at 24 weeks age.Polyp burden continuously increased but polyp number of small intestine had no more increase from the age of 9 weeks to 24 weeks.The oncogene of cyclinD1 was activated after the nucleic localization of β-catenin in adenomas.Conclusion APCMin/＋ mice is a good mouse model for the study of early stage of colorectal cancer.

关 键 词：结直肠肿瘤 APCMin/+小鼠 腺瘤 病理变化 

分 类 号：R361[医药卫生—病理学] R735.3[医药卫生—基础医学]