加味补阳还五汤对兔髂动脉球囊损伤后血管狭窄的影响及机制  

Effects of Jiawei Buyang Huanwu Decoction on Vascular Stenosis After Balloon Injury of Rabbit Iliac Artery

作　　者：周彬[1] 刘勇[1] 王敏[1] 吴琳[1] 廖火成[1] 刘定辉[1] 吴伟康[2] 钱孝贤[1,2] 

机构地区：[1]中山大学附属第三医院心血管内科,广东省广州市510630 [2]中山大学附属第三医院中西医结合研究所,广东省广州市510630

出　　处：《中国动脉硬化杂志》2011年第2期95-99,共5页Chinese Journal of Arteriosclerosis

基　　金：广州市科技计划项目(2001-J-019-01)

摘　　要：目的观察加味补阳还五汤对兔髂动脉球囊损伤后血管狭窄以及一氧化氮系统的影响。方法 24只4个月龄雄性新西兰兔随机分为假手术组、模型组和药物组,每组8只。假手术组兔予普通饲料,模型组和药物组兔予以高脂饮食。饲养2周后模型组和药物组兔行髂动脉内膜剥脱术,假手术组兔行假手术。药物组添加加味补阳还五汤药颗粒剂2 mL/(kg.d),假手术组及模型组喂食同前。观察4周后处死动物,通过普通光镜观察髂动脉内膜的损伤情况,并检测血清一氧化氮及一氧化氮合酶的水平;免疫组织化学法和逆转录聚合酶链反应法分别检测内皮型一氧化氮合酶蛋白和mRNA在血管的表达。结果病理观察可见假手术组兔髂动脉管壁光滑,管腔和管壁厚度正常;模型组管壁和内膜明显增厚、管腔明显狭窄,可见巨大动脉硬化斑块;而药物组管壁及内膜增厚较轻,管腔狭窄较轻。药物组兔血清一氧化氮水平和总一氧化氮合酶活性均明显高于模型组;免疫组织化学发现药物组内皮型一氧化氮合酶的蛋白和mRNA表达较模型组均明显增强。结论加味补阳还五汤具有防止家兔球囊扩张损伤髂动脉所致的管腔狭窄及抗实验性动脉粥样硬化作用,其机制可能与激活一氧化氮系统有关。Aim To investigate the protective effects of Jiawei Buyang Huanwu decoction on vascular stenosis and on the nitric oxide（NO） system after iliac artery were injured by balloon in diet-induced atherosclerotic rabbits. Methods 24 male New Zealand albino rabbits were equally randomized into control group,model group and drug group. The iliac arteries of the rabbits in the latter two groups were subjected to balloon injury. Four weeks later,serum NO synthases（NOS） activity and endothelial nitric oxide synthase（eNOS） level was assayed,endothelial hyperplasia,eNOS protein and mRNA expression were observed in injured iliac artery. Results Optical microscope revealed narrowed vascular lumen,thicken intima and numerous arteriosclerotic plaques in the model group compared with the control group,whereas the vascular lumen and intima thickness remained basically normal in drug group.The serum NO level and total NOS activity were higher in drug group than that of model group.Immunohistochemistry and RT-PCR results showed that eNOS protein and mRNA expression was stronger in rabbit iliac artery of drug group than that in model group. Conclusion Jiawei Buyang Huanwu decoction can lessen intimal hyperplasia and vascular stenosis in iliac artery injury rabbits,and this effect is possibly related with that Jiawei Buyang Huanwu decoction activated the NO system.

关 键 词：加味补阳还五汤 再狭窄 一氧化氮 

分 类 号：R363[医药卫生—病理学]