烟草烟雾对大鼠脑血管内皮细胞白细胞介素10表达的影响  

Expression of Interleukin-10 on Cerebral Vascular Endothelial Cells in Smoking Rats

作　　者：刘菲[1] 柳宗兰[2] 王一沙[1] 

机构地区：[1]沈阳医学院沈洲医院神经内科,辽宁省沈阳市110002 [2]中国医科大学附属第一医院神经内科,辽宁省沈阳市110001

出　　处：《中国动脉硬化杂志》2011年第2期125-127,共3页Chinese Journal of Arteriosclerosis

摘　　要：目的观察不同吸烟量大鼠脑血管内皮细胞白细胞介素10的表达,探讨烟草烟雾导致脑缺血的发病机制。方法健康Wistar大鼠60只,分为正常对照组、假暴露组、暴露卷烟烟气30天组、暴露卷烟烟气30天后停止暴露30天组(戒烟组)、暴露卷烟烟气90天高剂量组、暴露卷烟烟气90天低剂量组。通过免疫组织化学法观察大鼠脑血管内皮细胞白细胞介素10的表达。结果正常对照组和假暴露组白细胞介素10的表达较多,暴露卷烟烟气90天高剂量组白细胞介素10的表达明显低于其他各被动吸烟组,戒烟组白细胞介素10的表达明显高于其他各被动吸烟组,各被动吸烟组白细胞介素10的表达较正常对照组和假暴露组减少(P<0.05)。结论烟草烟雾可致脑血管内皮细胞白细胞介素10的表达减少,可能在缺血性脑血管病的发病中起重要作用。Aim To observe the expression of interleukin-10（IL-10） on cerebral vascular endothelial cells in smoking rats,and to discuss the mechanism of ischemia in brain caused by smoking. Methods 60 health Wistar rats were divied into 6 groups： normal control group,sham exposure group,exposed cigarette smoke 30 days group,exposed cigarette smoke 30 days then stopping exposion for 30 days group,exposed cigarette smoke 90 days high dose group and exposed cigarette smoke 90 days low dose group. The expression of IL-10 were observed in smoking rats by immunohistochemistry stain. Results The expression of IL-10 in normal control group and sham exposure group were more. The expression of IL-10 in exposed cigarette smoke 90 days high dose group were significantly lower than those in the other passive smoking groups. The expression of IL-10 in exposed cigarette smoke 30 days then stopping exposure for 30 days group were significantly higher than those in the other passive smoking groups. The expression of IL-10 in the passive smoking groups were significantly lower than those in control group and the sham exposure group（P0.05）. Conclusions Smoking can decrease IL-10 expression in endothelial cell,which plays an important role in ischemic cerebral vascular disease.

关 键 词：白细胞介素10 吸烟 血管内皮细胞 

分 类 号：R741[医药卫生—神经病学与精神病学]