夜间低温胁迫对番茄叶片活性氧代谢及AsA-GSH循环的影响  

Active Oxygen Metabolism and Ascorbate-Glutathione Cycle of Tomato Leaves under Low Nocturnal Temperature

作　　者：刘玉凤[1] 李天来[1] 高晓倩[1] 

机构地区：[1]沈阳农业大学园艺学院设施园艺省部共建教育部重点实验室辽宁省设施园艺重点实验室,沈阳110866

出　　处：《西北植物学报》2011年第4期707-714,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家重点基础研究发展计划"973"(2009CB119000);辽宁省重大科技攻关项目(2010215003);辽宁省教育厅优秀人才基金(2007R40)

摘　　要：以番茄品种‘辽园多丽’为试材,利用人工气候室模拟设施生产中的夜间低温胁迫环境,研究9℃和6℃夜低温对番茄叶片活性氧代谢和AsA-GSH循环的影响。结果显示:9℃和6℃夜间低温胁迫3～9d可诱导番茄叶片中超氧阴离子(O2.-)产生速率、过氧化氢(H2O2)和丙二醛(MDA)含量上升;抑制过氧化物酶(POD)、过氧化氢酶(CAT)的活性,增加超氧化物歧化酶(SOD)和AsA-GSH循环中抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)的活性,并提高还原型抗坏血酸(AsA)、还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)的含量。研究表明,在夜间低温胁迫过程中,增加的番茄叶片中SOD活性和AsA-GSH循环清除活性氧的能力并未与氧还原的速率一致,从而导致番茄叶片中活性氧的累积,使细胞膜系统受到一定破坏,在6℃处理的植物中尤为明显。The effect of low nocturnal temperature at 9℃ and 6℃ simulated by climate chambers on the active oxygen metabolism and Ascorbate-Glutathione cycle of tomato leaves was studied.The results showed that low nocturnal temperature treatment increased O-·2 production rate,H2O2,malondialdehyde（MDA） contents;inhibited peroxidase（POD）,catalase（CAT） activities;and improved superoxide dismutase（SOD）,ascorbate peroxidase（APX）,dehydroascorbate reductase（DHAR）,glutathione reductase（GR） activities as well as reduced-ascorbate（AsA）,reduced-glutathione（GSH）,oxidized-glutathione（GSSG） contents.It indicated that the enhanced SOD activity and scavenging activity of Ascorbate-Glutathione cycle did not keep pace with the rate of oxygen reduction since increased O-·2,H2O2 and MDA levels were observed throughout the experiment,especially in 6℃ treated plants.

关 键 词：番茄 夜间低温 活性氧代谢 AsA-GSH循环 

分 类 号：Q945.78[生物学—植物学]