GMPase超表达对番茄植株抗坏血酸含量及耐冷性相关生理指标的影响  

Effects of GMPase Overexpression on Ascorbic Acid Content and Relative Index to Low-temperature Tolerance in Tomato Plants

作　　者：李超汉[1] 张琳[1] 史庆华[1] 李青竹[1] 郭晓青[1] 李霞[1] 于贤昌[2] 

机构地区：[1]山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安271018 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《园艺学报》2011年第4期692-700,共9页Acta Horticulturae Sinica

基　　金：国家重点基础研究发展计划项目(2009CB119000);国家自然科学基金项目(30771473);现代农业产业技术体系建设专项(Nycytx-35GW21);农业部园艺作物遗传改良重点开放实验室资助项目

摘　　要：采用农杆菌介导法获得了GDP-D-甘露糖焦磷酸化酶基因(GMPase)超表达的转基因番茄植株,并且采用Western blot检测到有两株GMPase蛋白表达显著增加。比较了这两个株系和野生型植株中抗坏血酸含量及耐低温能力。与野生型番茄相比,GMPase超表达在常温及低温胁迫下均使GMPase活性提高,使AsA和DHA含量显著增加,降低了低温胁迫下膜脂过氧化产物MDA的积累。Two transgenic tomato plants of overexpressing GDP-D-mannose pyrophosphorylase gene （GMPase）were obtained by Agrobacterium-mediated transformation,and Western blot analysis indicated that the GMPase protein in transgenic plants was significantly increased.T0 make sure the function of GMPase overexpression,ascorbate content and cold tolerance capacity were studied.Compared with wild-type plants,the GMPase activity and contents of AsA and DHA in both normal and low-temperature conditions were increased and the MDA content was decreased in the transgenic plants.Based on these results,it can be concluded that the overexpression of GMPase may increase tomato tolerance to cold stress by increasing AsA level and lowering lipid peroxidation.

关 键 词：番茄 抗坏血酸 GDP–D–甘露糖焦磷酸化酶 转基因 低温胁迫 

分 类 号：S641.2[农业科学—蔬菜学]