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作 者:陶丹丹[1] 徐洁洁[1] 陈仁杰[2] 毛圆[2] 刘根焰[3] 乔明哲[1] 陈曦[1] 陆美萍[1] 丁竞竞[4]
机构地区:[1]南京医科大学第一附属医院耳鼻咽喉科,南京210029 [2]南京医科大学第二附属医院耳鼻咽喉科 [3]南京医科大学第一附属医院检验科 [4]南京医科大学公共卫生学院
出 处:《临床耳鼻咽喉头颈外科杂志》2011年第9期401-405,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基 金:江苏省"科教兴卫工程"医学重点学科研究基金(No:XK200719);江苏省人事厅"六大人才高峰"资助项目(No:07-B-037)
摘 要:目的:观察EB病毒(EBV)潜伏膜蛋白1(LMP1)抗体在鼻咽癌(NPC)患者血清中的水平,探讨LMP1抗体检测在NPC血清学诊断、预后判断及肿瘤靶向免疫治疗中的临床意义。方法:采用ELISA及West-ern blot方法,以LMP1融合蛋白检测61例NPC患者(NPC组)、30例鼻咽炎患者(鼻咽炎组)和55例正常人(对照组)血清中LMP1抗体水平。分析LMP1抗体水平与NPC临床病理特征的关系。结果:ELISA方法证实,NPC组LMP1抗体水平明显高于鼻咽炎组及对照组,鼻咽炎组LMP1抗体水平明显高于对照组,组间均差异有统计学意义(均P<0.05)。NPC组LMP1抗体水平在NPC不同病理分级及有无淋巴结转移中均差异无统计学意义(均P>0.05)。Western blot方法表明,NPC组LMP1抗体的表达均较鼻咽炎组及对照组高。结论:NPC组EBV LMP1抗体水平显著高于鼻咽炎组及对照组,LMP1融合蛋白可考虑作为肿瘤抗原标志物辅助NPC血清学诊断及肿瘤靶向免疫治疗。Objective:To detect the levels of Epstein-Barr virus(EBV) latent membrane protein 1 Antibodies(LMP1-Ab) in nasopharyngeal carcinoma(NPC)sera and discuss the clinical significance of this test in diagnosis,prognosis,and immune-targeted therapy of NPC.Method:Enzyme-linked immunosorbent assay(ELISA) and Western blot method were used to detect the LMP1-Ab levels in 61 NPC sera,30 nasopharyngitis sera,and 55 normal sera.The relationship between the LMP1-Ab level and clinical and pathological features of NPC was analyzed.Result:ELISA test showed that LMP1 antibodies level was significantly higher in nasopharyngeal carcinoma group than those in nasopharyngitis group and in healthy group and there were statistical significances(all P0.05).In NPC group,the LMP1-Ab level was not related to the pathological grade and cervical lymph node metastases(P0.05).Western blot test revealed that the expression of LMP1 antibodies was higher in NPC sera than in nasopharyngitis sera and in normal sera.Conclusion:LMP1-Ab level was higher in nasopharyngeal carcinoma group than in nasopharyngitis group and in normal group.Therefore,LMP1 may be considered as a tumor correlated antigen to help the diagnosis and immune-targeted therapy of NPC.
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