人肺癌高转移细胞系的转移相关基因观察  

Observation on Metastasis-related Genes of Highly Metastatic Human Lung Cancer Cell Line

作　　者：姚明[1,2] 李静[2] 英德水[2] 张方琳[1] 闫明霞[2] 刘蕾[2] 张书霞[1] 

机构地区：[1]南京农业大学动物医学院,南京210095 [2]上海市肿瘤研究所实验病理研究室,上海200032

出　　处：《实验动物与比较医学》2011年第2期79-82,共4页Laboratory Animal and Comparative Medicine

基　　金：上海市科委创新行动计划（09140902000,10140902401）

摘　　要：目的 观察人肺癌高转移细胞系SPC-A-1sci的转移相关基因表达,同时验证该细胞系的体内高转移特性.方法 通过Western blot,FICC、IHC等方法检测转移相关基因CD44,OPN,MMP-9,EGFR在高、低转移细胞系间的差异表达;采用尾静脉注射方法观察高、低转移细胞系的转移特性.结果 与低转移细胞系相比,转移促进基因CD44,OPN,MMP-9,EGFR在高转移细胞中的表达均显著上调.体内实验表明,8周时高转移细胞系的肺转移率为100%（12/12）,而低转移细胞系的肺转移率仅为16.67%（2/12）.结论 人肺癌高转移细胞系SPC-A-1sci在转移相关基因表达和动物体内均表现出典型的高转移特性。Objective To observe the metastasis-associated genes expression in the highly metastatic human lung cancer cell line SPC-A-lsci and validate the highly metastatic characteristics of SPC-A-1 sci in vivo. Methods The expression levels of several metastasis promoting genes concluding CD44, OPN, MMP-9, EGFR were analyzed between the high and low metastatic cell lines by RT-PCR, Western blotting, FICC, IHC and so on. The metastatic characteristics was investigated by injection the cell lines into the tall vein of nude mice. Results Compared with the low metastatic cell lines, the metastasis- associated genes CD44, OPN, MMP-9, EGFR in highly metastatic cells were significantly increased. After 8 weeks in vivo experiments, 100% （12/12） of mice injected with highly metastatic cell lines developed lung metastases, while low metastatic cell lines was 16.67%（2/12）. Conclusions The metastasis-associated genes are highly expressed in SPC-A-lsci, and the SPC-A-lsci shows the typical characteristics of highly metastatic in vivo, and may be served as a reference for the application of the cell lines.

关 键 词：肺癌 细胞株 肿瘤转移 转移相关基因 

分 类 号：R734.2[医药卫生—肿瘤] Q95-33[医药卫生—临床医学]