南瓜蛋白对人胰腺癌SW1990细胞株的诱导凋亡作用  

Effects of Cucurmosin on Apoptosis in Human Pancreatic Cancer Cell Line SW1990

作　　者：殷强[1] 黄鹤光[1] 谢捷明[2] 陈明晃[3] 张宝明[1] 王丛菲[1] 

机构地区：[1]福建医科大学附属协和医院普外科,福建福州350001 [2]福建医科大学药学院药理学系,福建福州350004 [3]中国科学院福建物质结构研究所结构化学国家重点实验室,福建福州350002

出　　处：《中国普外基础与临床杂志》2011年第4期380-383,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：福建医科大学重大科研项目(项目编号:09ZD012);福建省自然科学基金资助项目(项目编号:C0510012)~~

摘　　要：目的探讨南瓜蛋白体外对人胰腺癌SW1990细胞株的诱导凋亡作用。方法 MTT法检测南瓜蛋白对SW1990细胞的增殖抑制作用,透射电镜观察细胞凋亡表现,流式细胞仪检测其凋亡率,Western blot法检测caspase-3蛋白的表达。结果不同浓度(1.25、2.50、5.00、10.00、20.00、40.00及80.00μg/ml)南瓜蛋白作用不同时间(24、48及72 h)后,南瓜蛋白对胰腺癌细胞具有生长抑制作用,并呈时间和剂量依赖性(P<0.05)。40.00μg/ml南瓜蛋白作用72 h后,透射电镜下可见细胞出现明显的凋亡改变,并可见凋亡小体;不同浓度(0、2.50、10.00、40.00μg/ml)南瓜蛋白作用72 h后,细胞的凋亡率分别为(0.30±0.11)%、(18.93±1.06)%、(28.00±2.07)%及(49.93±3.25)%,随着药物浓度的升高,细胞的凋亡率逐渐增加,呈浓度依赖性(P<0.05);caspase-3蛋白表达随着药物浓度的升高逐渐增强,呈浓度依赖性(P<0.05)。结论南瓜蛋白可能通过上调caspase-3蛋白的表达诱导胰腺癌细胞凋亡。Objective To investigate the possible mechanism of cucurmosin on apoptosis in human pancreatic cancer cell line SW1990 in vitro. Methods The inhibition of cucurmosin on SW1990 cell was detected by MTT as-say, the apoptosis was observed by transmission electron microscope, the apoptosis rate was analyzed by flow cy-tometry, and the protein level of caspase-3 was determined by Western blot. Results After exposure to cucurmosin at 1.25, 2.50, 5.00, 10.00, 20.00, 40.00, and 80.00μg/ml for 24, 48, and 72 h, the proliferation of SW1990 celt was inhibited in a time- and dose-dependent manner （P〈0.05）. At 72 h after 40.00 /μg/ml cucurmosin treat- ment, the typical apoptosis changes and apoptotic bodies were observed by transmission electron microscope. After exposure to cucurmosin at 0, 2.50, 10.00, and 40.00 μg/ml for 72 h, the apoptosis rate increased gradually as （0.30±0.11）%, （18.93±1.06）%, （28. 00±2.07）%, and （49. 93±3.25）%, respectively （P〈0.05）. The ex- pression of caspase-3 protein was elevated gradually （P〈0.05）. Conclusion Cucurmosin may induce the apoptosis of pancreatic cancer cell through up-regulating the expression of caspase-3.

关 键 词：胰腺肿瘤 南瓜蛋白 凋亡 

分 类 号：R735.9[医药卫生—肿瘤]