NOV及BNIP3基因在小鼠粒单核细胞白血病中的表达及其意义  

Expression of NOV and BNIP3 Gene in Mouse Myelomonocytic Leukemia and Its Significance

作　　者：左洪莉[1] 彭恩兰[1] 赵红霞[1] 孙雪冬[1] 郭梅[1] 王丹红[1] 乔建辉[1] 孙琪云[1] 余长林[1] 胡凯勋[1] 杨啊晶[2] 艾辉胜[1] 

机构地区：[1]军事医学科学院附属医院血液科,北京100071 [2]北京大学第一医院,北京100871

出　　处：《中国实验血液学杂志》2011年第2期293-297,共5页Journal of Experimental Hematology

摘　　要：本研究探讨NOV及BNIP3基因在小鼠粒单核细胞白血病中的表达及意义。给小鼠经尾静脉接种小鼠粒单核细胞白血病细胞株WEHI-3,随机将小鼠分成化疗组和对照组,然后在接种后不同时间点取2组小鼠骨髓组织,采用qRT-PCR方法动态检测NOV、BNIP3 mRNA的表达情况,分析其表达与白血病发生及发展的关系。结果表明:对照组小鼠NOV、BNIP3 mRNA表达水平在接种后2周开始逐渐增高,死亡时达到高峰,分别由接种前的1.85E-05与3.44E-03,升高至濒死时的3.57E-02与3.48E-02(p<0.05)。化疗组小鼠2种基因的表达率则在化疗后迅速降至2.51E-05与1.58E-03(p<0.05),与接种前水平相近(p>0.05),复发时再次升高,死亡时2基因表达率与对照组无明显差异(p>0.05)。结论:NOV及BNIP3基因在白血病小鼠中表达明显增高,其表达异常可能参与了小鼠白血病的发生与发展;2种基因表达率与疗效密切相关,因此NOV和BNIP3表达水平在疗效评估及MRD检测中具有重要价值。This study was aimed to investigate the expression level of NOV and BNIP3 mRNA in mice myelomonocytic leukemia（AML-M4） and its significance.The mice were inoculated intravenously with myelomonocytic leukemia cells of WEHI-3,and divided randomly into chemotheraphy group and control（untreated） group.Bone marrow samples were then collected from both groups at different times.The NOV and BNIP3 mRNA expression were detected by TaqMan quantitative real-time reverse transcription polymerase chain reaction（qRT-PCR）,and the relationship between these expression levels and clinical significance in leukemia incidence and progression were analyzed with β-actin as the housekeeping gene.The results showed that the mean values of NOV and BNIP3 increased gradually from 2 weeks after inoculation and achieved highest level at death in control group.Expression level of NOV increased from 1.85E-05 before inoculation to 3.57E-02 at death（p〈0.05）,and BNIP3 from 3.44E-03 to 3.48E-02.While 2 gene expression in the chemotherapy group decreased quickly to 2.51E-05 and 1.58E-03（p〈0.05）respectively after chemotheraphy,which were close to the level before inoculation（p〉0.05）.The 2 gene expressions again rose at relapse,and difference of expression level between 2 group at death were no statistically significant（p〉0.05）.It is concluded that the expression of NOV and BNIP3 in leukemia AML-M4 is significantly higher than that in normal controls,of which high level expression is an important factor in the development of leukemia.Close relation between the therapeutic effect and expression level of these two genes suggests the great value in prognostic evaluation and MRD detection.

关 键 词：NOV基因 BNIP3基因 粒单核细胞白血病 

分 类 号：R733.7[医药卫生—肿瘤]