尾型同源盒基因在成人急性淋巴细胞白血病中表达及临床意义  

Expression and Clinical Significance of Cadual Type Homeobox Transcription Factor-2 in Adult Acute Lymphocytic Leukemia

作　　者：鲁海艳[1] 夏海龙[1] 陈晓文[1] 朱立新[1] 王庆义[1] 程歆[1] 

机构地区：[1]安徽医科大学第一附属医院血液科,安徽合肥230022

出　　处：《中国实验血液学杂志》2011年第2期298-302,共5页Journal of Experimental Hematology

摘　　要：本研究旨在探讨同源盒基因CDX1,CDX2和CDX4在成人急性淋巴细胞白血病(ALL)中的表达及其临床意义。急性淋巴细胞白血病骨髓或外周血标本51份,采用逆转录聚合酶链反应(RT-PCR)检测CDX1,CDX2和CDX4在成人急性淋巴细胞白血病患者和14例正常对照组中的表达情况。结果表明,在14例健康成人中未见CDX1,CDX2和CDX4表达,15例缓解期急性淋巴细胞白血病患者中未见CDX1,CDX2和CDX4表达,在初治ALL患者中CDX2的表达率为60.8%,而在治疗缓解后其表达率明显下降(p<0.05);在复发组患者中CDX2表达率又升高(81.8%);CDX2表达与疾病危险分组相关,高危组CDX2阳性率为91.7%,高于标危组45.7%(p<0.05);CDX1和CDX4在初治及复发成人ALL中皆无表达。在初治和复发的成人ALL患者中CDX2表达阳性患者的完全缓解率(CR)低于表达阴性的患者(p<0.05);CDX2表达阳性患者的中位生存时间短于阴性表达的患者。结论:CDX2表达和成人ALL的发病、复发相关,CDX1和CDX4表达与成人ALL发病及复发无相关性;CDX2表达阳性的患者完全缓解率低,预后不良;CDX2可作为成人急性淋巴细胞白血病的发病、复发及预后判断的指标之一。This study was aimed to investigate the expression and clinical significance of CDX1,CDX2 and CDX4 genes in acute lymphocytic leukemia（ALL）.Expressions of CDX1,CDX2,and CDX4 in 51 adult acute lymphocytic leukemia patients and 14 healthy subjects were detected by reverse transcription polymerase chain reaction（RT-PCR）.The results indicated that CDX1,CDX2 and CDX4 were not expressed in 14 healthy persons and 15 CR ALL patients,the positive expression rate of CDX2 gene in de novo ALL patients was 60.8%,while it obviously decreased in patients with complete remission（CR）（p 0.05）;the expression of CDX2 was increased again in relapsed patients（81.8%）.When the expression of CDX2 was analyzed in different risk groups of ALL patients,the CDX2 expression rate in high risk（HR） patients was 91.7%,and that in the standard risk（SR） group was 45.7%.Furthermore,analyses of CDX1 and CDX4 expression in series of ALL samples did not show the expression of these genes.In patients with adult ALL at diagnosis and relapse,the CR rate of patients with CDX2 positive expression was lower than that of patients with CDX2 negative expression（p〈0.05）.The median survival time in CDX2 positive expression patients was shorter than that in negative expression patient.It is concluded that expression of CDX2 may correlated with pathogenesis and relapse of adult ALL,but the expression of CDX1 and CDX4 don＇ t associated with pathogenesis and relapse of adult ALL;the CR rate and prognosis of patients with CDX2 possitive expression is lower and poor.The expression of CDX2 may be used as a marker for occurrence,relapse and poor prognosis of adult ALL patients.

关 键 词：尾型同源盒基因 CDX1基因 CDX2基因 CDX4基因 急淋巴细胞白血病 

分 类 号：R733.1[医药卫生—肿瘤]