SDF-1/CXCR4在小儿急性白血病中的表达及其意义  

Level of SDF-1/CXCR4 in Children with Acute Leukemia and Its Significance

作　　者：温泉[1] 陈日玲[1] 蔡康荣[2] 林永文[1] 

机构地区：[1]广东医学院附属医院儿科,广东湛江524001 [2]广东医学院中心实验室,广东湛江524023

出　　处：《中国实验血液学杂志》2011年第2期324-326,共3页Journal of Experimental Hematology

摘　　要：本研究探讨儿童急性白血病(AL)血浆基质细胞衍生因子-1(SDF-1)的水平和骨髓细胞SDF-1受体CXCR4的表达与髓外浸润的关系。分别收集48例AL患儿、20例非恶性血液病患儿(对照组)外周血浆及骨髓细胞,采用ELISA法分别检测AL患儿和对照组儿童外周血浆SDF-1水平;用流式细胞术检测AL患儿和对照组儿童骨髓细胞CXCR4的表达。结果表明:血浆SDF-1水平及骨髓细胞CXCR4表达,在AL组明显高于对照组(p<0.001),在急性淋巴细胞白血病(ALL)组明显高于急性髓细胞白血病(AML)组(p<0.01),在髓外浸润组也高于非髓外浸润组(p<0.05)。结论:SDF-1和CXCR4在AL儿童呈高水平表达,与白血病的类型、骨髓白血病细胞的迁移、浸润密切相关。The objective of this study was to detect the level of plasma stromal cell-derived factor-1（SDF-1） and the expression of CXCR4（SDF-1 receptor in bone marrow cells） in children with Acute Leukemia（AL） and to investigate the relationship between the expression of CXCR4 and extramedullary infiltration.48 children with acute leukemia and 20 with non-hematologic malignancies were selected into the AL group and the control goroup respectively.The peripheral plasma and bone marrow cells were collected.The level of SDF-1 in peripheral plasma was detected by ELISA and the expression of CXCR4 in bone marrow cells was determined by flow cytometry.The results showed that the levels of SDF-1 in peripheral plasma and the expression of CXCR4 in bone marrow cells of AL group was significantly higer than that of control group,among which the level of SDF-1 of the acute lymphoblastic leukemia（ALL） group was also higher than that of the acute myeloid leukemia（AML） group,the expression level of CXCR4 in the bone marrow cells of the extramedullary infiltration（EI） group was higer than that of the non-extramedullary Infiltration（NI） group,and all the differences between the both groups were significant.It is concluded that SDF-1 and CXCR4 express a high level in children with AL,which closely relates with the type of leukemia and the migration and inflitration of leukemia cells in bone marrow.

关 键 词：SDF-1 CXCR4 急性白血病 

分 类 号：R733.71[医药卫生—肿瘤]