多发性骨髓瘤患者BAFF与BCL-2 mRNA的表达及意义  

Expression and Significance of B Cell-activating Factor of TNF Family(BAFF) and B Cell Lymphoma/Leukemia-2(BCL-2) in Multiple Myeloma

作　　者：王松梅[1] 张腾龙[1] 蒋玉梅[1,2] 吴弘英[1] 郝鲁梅[1] 邢秀华[1] 

机构地区：[1]青岛大学医学院附属青岛市立医院东院血液科,山东青岛266071 [2]山东烟台市食品药品监督局

出　　处：《中国实验血液学杂志》2011年第2期395-398,共4页Journal of Experimental Hematology

基　　金：山东省青岛市公共领域科技支撑计划(编号2010KZJ-9)

摘　　要：本研究探讨B细胞活化因子(BAFF)和B细胞淋巴瘤/白血病-2基因(BCL-2)mRNA在多发性骨髓瘤(MM)患者骨髓单个核细胞(BMMNC)中表达水平及BAFF和BCL-2对MM的发生、发展及预后的意义。收集40例MM患者及10例正常对照的骨髓标本,获取BMMNC;采用实时荧光定量PCR(real time PCR)的方法检测BMMNC中BAFF与BCL-2 mRNA的表达,并同步收取血浆,检测β2-MG分泌水平;按Durie-Salmon(D-S)分期标准对MM患者进行临床分期。结果表明,①BAFF与BCL-2 mRNA在MM组中表达升高,与对照组比较差异有统计学意义,治疗后平台期BAFF与BCL-2 mRNA表达水平明显减低(p<0.05);②复发/难治性多发性骨髓瘤患者BAFF与BCL-2 mRNA的表达水平较正常对照组及平台期患者明显升高(p<0.05),初发组与复发/难治组之间表达无显著差异。BAFF与BCL-2 mRNA表达与患者病程D-S分期和β2-MG水平有相关性。结论:MM患者BAFF与BCL-2mRNA表达水平升高,并且MM初治组、复发/难治组BAFF与BCL-2 mRNA表达水平均高于平台组,提示BAFF和BCL-2可能参与MM的发生、发展的病理机制;BAFF与BCL-2 mRNA表达水平与MM肿瘤负荷呈正相关,提示BAFF和BCL-2表达水平可以作为评判MM患者预后的新指标。This study was aimed to explore the expression of B cell-activating factor of TNF family（BAFF） and B cell lymyhoma/leukemia-2（BCL-2） in bone marrow mononuclear cells（BMMNC） of multiple myeloma（MM） and the significance of BAFF and BCL-2 for occurrence,development and prognosis of MM.The bone marrow of 40 cases of MM and 10 healthy persons was collected,the mononuclear cells（MNC） were isolated,the expression of BAFF and BCL-2 mRNA in BMMNC was detected by real-time PCR;the plasma was simultaneously collected and the β2-MG level was determined;the clinical staging of MM patients was performed according to Durie-Salmon（D-S） staging criterion.The results indicated that the expression level of BAFF and BCL-2 mRNA in MM patients increased,as compared with normal controls,the difference was statistical significant（p〈0.05）;the expression level of BAFF and BCL-2 mRNA in plateau stage after treatment obviously decreased.The expression level of BAFF and BCL-2 mRNA in relapsed/refractory MM patients was significanty higher than that in normal controls and patients reached plateau stage（p〈0.05）,there was no statistically significant difference between newly diagnosed and relapsed/refractory MM patients（p〉0.05）.The expression of BAFF and BCL-2 mRNA related with D-S staging and β2-MG level.It is concluded that the expression levels of BAFF and BCL-2 mRNA increase,morever the expression levels of BAFF and BCL-2 mRNA in newly diagnosed and relapsed/refractory MM patients are higher than those in patients reached plateau stage,which suggest the BAFF and BCL-2 may be involved in occurrence and development of MM;the relation of expression level of BAFF and BCL-2 mRNA to MM load is positive,which indicates the expression level of BAFF and BCL-2 mRNA may be a new indicator for evatuating the prognosis of MM patients.

关 键 词：B细胞活化因子 B细胞淋巴瘤/白血病-2基因 多发性骨髓瘤 

分 类 号：R733.3[医药卫生—肿瘤]