增生性贫血患儿骨髓单个核细胞转铁蛋白受体2表达情况及其意义探讨  

TfR2 mRNA Expression in Bone Marrow Mononuclear Cells of Children with Hyperplastic Anemia and Its Implications

作　　者：陈婷婷[1] 袁粒星[2] 潘玲丽[1] 马志贵[3] 顾玲[3] 朱易萍[3] 高举[3] 

机构地区：[1]四川大学华西第二医院急诊科,成都610041 [2]四川大学华西第二医院公共实验室,成都610041 [3]四川大学华西第二医院血液儿科,成都610041

出　　处：《中国实验血液学杂志》2011年第2期439-443,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金(编号30973237);四川省科技厅应用基础项目资助(编号2010JY0004)资助完成

摘　　要：本研究旨在探讨增生性贫血患儿骨髓单个核细胞转铁蛋白受体2(transferrin receptor 2,TfR2)表达情况,分析TfR2 mRNA表达水平与贫血程度、骨髓红系增生程度、基础疾病种类和铁状况等指标的相关关系,评价TfR2受体蛋白在红系造血方面的作用和在增生性贫血诊断方面的应用价值。实验分为两组:实验组系我院儿童血液肿瘤科收治的40例增生性贫血患者,对照组系经骨髓检查排除红系疾病和血液系恶性肿瘤的10例患者。收集研究对象的骨髓抗凝标本,采用荧光定量PCR检测增生性贫血患儿骨髓单个核细胞TfR2 mRNA表达水平,分析其与骨髓增生程度、原发疾病种类以及机体铁状况的关系。结果表明,增生性贫血组骨髓单个核细胞TfR2相对表达量显著高于对照组;增生性贫血组TfR2相对表达量与外周血Hb含量呈负相关关系(rs=-0.715),而与骨髓幼红细胞比例呈正相关关系(rs=0.533)。结论:增生性贫血患者TfR2表达水平增高,与骨髓增生程度、外周血贫血程度密切相关。The aim of this study was to investigate the expression of transferrin receptor 2（TfR2） mRNA in bone marrow mononuclear cells（BMMNC） of children with hyperplastic anemia（HA）,to analyze the correlation of TfR2 mRNA expression level with Hb level,bone marrow erythroid hyperplasia,iron status in body and underlying diseases,and to evaluate the role of TfR2 in erythroid hemopoiesis and the useful value in diagnosis of HA.The experiment was divided into 2 groups： test group,in which 40 patients with HA were enrolled,and control group in which 10 patients without erythroid disorders and hematological malignancies confirmed by bone marrow examination were enrolled.The bone marrow samples of patients in mentioned above 2 groups were collected,the TfR2 mRNA expression in BMMNC of patients with HA was detected by fluorescence-quantitative PCR,the correlation of HA with bone marrow erythroid hyperplasia,iron status of body and underlying diseases was analyzed.The results showed that the relative level of TfR2 mRNA expression in HA patients was significantly higher than that in control patients.The TfR2 mRNA expression level negatively correlated with Hb level in peripheral blood（r=-0.715）,while it positively correlated with ratio of bone marrow erythroblasts（r=0.533）.It is concluded that TfR2 mRNA expression in HA patients increases and closely correlates with hyperplasia status of bone marrow and anemia level in peripheral blood.

关 键 词：转铁蛋白受体2 增生性贫血 红系造血 骨髓单个核细胞 

分 类 号：R556[医药卫生—血液循环系统疾病]