The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings  

作　　者：Yan SUN Chao-shu TANG Hong-fang JIN Jun-bao DU 

机构地区：[1]Department of Pediatrics, Peking University First Hospital, Beijing 100034, China [2]Institute of Cardiovascular Research, First Hospital of Peking University, Beijing 100034, China [3]Key Laboratory of Molecular Cardiology, Ministry of Education, Beijing 100083, China

出　　处：《Acta Pharmacologica Sinica》2011年第4期456-464,共9页中国药理学报（英文版）

基　　金：Acknowledgments This work was supported by the Foundation of the Ministry of Education, People＇s Republic of China （20070001702 and 20070001770）; the National Natural Science Foundation of China 81070212, 30821001, and 30801251）; the Major Basic Research Development Program of the People＇s Republic of China （2011CB503904）; and the Beijing Natural Science Foundation （7082095）. We thank Shu-xu DU and Wei LU for their technical assis- tance.

摘　　要：Aim： To compare the vasorelaxing effects of hydrogen sulfide （H2S） on isolated aortic and pulmonary artery rings and to determine their action mechanisms. Methods： H2S-induced vasorelaxation of isolated rat aortic versus pulmonary artery rings under 95%O2 and 5% COg was analyzed. The expression of cystathinonine gamma-lyase （CSE）, cystathionine beta synthase （CBS）, 3-mercaptopyruvate sulfurtransferase （3MST）, SUR2B and Kir6.1 was examined. Results： NariS caused vasorelaxation of rat aortic and pulmonary artery rings in a dose-dependent manner. NariS dilated aortic rings to a greater extent （16.4%, 38.4%, 64.1%, 84.3%, and 95.9% at concentrations of 50, 100, 200, 500, and 1000 pmol/L, respectively） than pulmonary artery rings （10.1%, 22.2%, 50.6%, 73.6%, and 84.6% at concentrations of 50, 100, 200, 500 and 1000 pmol/L, respectively）. The EC50 of the vasorelaxant effect for aortic rings was 152.17 μmol/L, whereas the EC50 for pulmonary artery rings was 233.65 μmol/L. The vasorelaxing effect of H2S was markedly blocked b y cellular and mitochondrial membrane KATP channel block-ers in aortic rings （P〈0.01）. In contrast, only the cellular membrane KATp channel blocker inhibited H2S-induced vasorelaxation in pulmonary artery rings. SUR2B mRNA and protein expression was higher in aortic rings than in pulmonary artery rings. Cystathinonine gamma-lyase （CSE） but not cystathionine beta synthase （CBS） expression in aortic rings was higher than in pulmonary artery rings. 3-Mercapto pyruvate sulfurtransferase （3MST） mRNA was lower in aortic rings than in pulmonary artery rings. Conclusion： The vasorelaxing effect of H2S on isolated aortic rings was more pronounced than the effect on pulmonary artery rings at specific concentrations, which might be associated with increased expression of the KATp channel subunit SUR2B.Aim： To compare the vasorelaxing effects of hydrogen sulfide （H2S） on isolated aortic and pulmonary artery rings and to determine their action mechanisms. Methods： H2S-induced vasorelaxation of isolated rat aortic versus pulmonary artery rings under 95%O2 and 5% COg was analyzed. The expression of cystathinonine gamma-lyase （CSE）, cystathionine beta synthase （CBS）, 3-mercaptopyruvate sulfurtransferase （3MST）, SUR2B and Kir6.1 was examined. Results： NariS caused vasorelaxation of rat aortic and pulmonary artery rings in a dose-dependent manner. NariS dilated aortic rings to a greater extent （16.4%, 38.4%, 64.1%, 84.3%, and 95.9% at concentrations of 50, 100, 200, 500, and 1000 pmol/L, respectively） than pulmonary artery rings （10.1%, 22.2%, 50.6%, 73.6%, and 84.6% at concentrations of 50, 100, 200, 500 and 1000 pmol/L, respectively）. The EC50 of the vasorelaxant effect for aortic rings was 152.17 μmol/L, whereas the EC50 for pulmonary artery rings was 233.65 μmol/L. The vasorelaxing effect of H2S was markedly blocked b y cellular and mitochondrial membrane KATP channel block-ers in aortic rings （P〈0.01）. In contrast, only the cellular membrane KATp channel blocker inhibited H2S-induced vasorelaxation in pulmonary artery rings. SUR2B mRNA and protein expression was higher in aortic rings than in pulmonary artery rings. Cystathinonine gamma-lyase （CSE） but not cystathionine beta synthase （CBS） expression in aortic rings was higher than in pulmonary artery rings. 3-Mercapto pyruvate sulfurtransferase （3MST） mRNA was lower in aortic rings than in pulmonary artery rings. Conclusion： The vasorelaxing effect of H2S on isolated aortic rings was more pronounced than the effect on pulmonary artery rings at specific concentrations, which might be associated with increased expression of the KATp channel subunit SUR2B.

关 键 词：hydrogen sulfide aortic rings pulmonary rings VASORELAXATION cystathinonine gamma-lyase cystathionine beta synthase 3-mercapto pyruvate sulfurtransferase glibenclamide  5-hydroxydecanoate 

分 类 号：Q959.837[生物学—动物学] S858.31[农业科学—临床兽医学]