Contractile effect of tachykinins on rabbit sma intestine  

作　　者：Marta Sofia VALERO Diego Santos FAGUNDES Laura GRASA Maria Pilar ARRUEBO Miguel Angel PLAZA Maria Divina MURILLO 

机构地区：[1]Departamento de Farmacologia y Fisiologia (Fisiologia), Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza (Spain)

出　　处：《Acta Pharmacologica Sinica》2011年第4期487-494,共8页中国药理学报（英文版）

摘　　要：Aim： To study the role of the tachykinin receptors in spontaneous contractions of longitudinal and circular smooth muscle from rabbit small intestine and to determine the mechanism of action of Substance P （SP）. Methods： Rabbit duodenum, jejunum and ileum segments were prepared. The spontaneous contractions of longitudinal and circular smooth muscle were recorded using a computer via an isometric force transducer. The specific agonists and antagonists of tachykinin receptors were added into the organ bath. Results： The agonists of tachykinin NK1 receptor （SP and [Sar9] SP）, NK2 receptor （NKA and （13-Ala8）-NKA）, and NK3 receptor （NKB and Senktide） all induced contractions in the small intestine. The contractions were diminished by NK1 receptor antagonist L-733,060 NK2 receptor antagonist GR-94800, and NK3 receptor antagonist SB 218795. Contractions caused by SP were also reduced by atro-pine, verapamil, PKC inhibitor staurosporine, and PLC inhibitor U73122. Conclusion： Ttachykinin NK1, NK2, and NK3 receptors mediate the contractions of the smooth muscle in rabbit intestine. Furthermore SP acts directly on smooth muscle cells through the tachykinin NK1 receptor.Aim： To study the role of the tachykinin receptors in spontaneous contractions of longitudinal and circular smooth muscle from rabbit small intestine and to determine the mechanism of action of Substance P （SP）. Methods： Rabbit duodenum, jejunum and ileum segments were prepared. The spontaneous contractions of longitudinal and circular smooth muscle were recorded using a computer via an isometric force transducer. The specific agonists and antagonists of tachykinin receptors were added into the organ bath. Results： The agonists of tachykinin NK1 receptor （SP and [Sar9] SP）, NK2 receptor （NKA and （13-Ala8）-NKA）, and NK3 receptor （NKB and Senktide） all induced contractions in the small intestine. The contractions were diminished by NK1 receptor antagonist L-733,060 NK2 receptor antagonist GR-94800, and NK3 receptor antagonist SB 218795. Contractions caused by SP were also reduced by atro-pine, verapamil, PKC inhibitor staurosporine, and PLC inhibitor U73122. Conclusion： Ttachykinin NK1, NK2, and NK3 receptors mediate the contractions of the smooth muscle in rabbit intestine. Furthermore SP acts directly on smooth muscle cells through the tachykinin NK1 receptor.

关 键 词：tachykinin receptors substance P small intestine RABBITS STAUROSPORINE U73122 L-733 060 GR-94800 SB 218795 

分 类 号：S853.74[农业科学—临床兽医学] Q42[农业科学—兽医学]