Id-1在结直肠癌中的表达及其与bFGF、Bcl-2和PCNA的关系  

Expression of Id-1 in Colorectal Cancer and its Relationship with bFGF,Bcl-2 and PCNA

作　　者：刘豫瑞[1] 吴志贤[1] 曾达武[1] 林荆[1] 

机构地区：[1]福建医科大学附属第一医院肝病中心,福州350005

出　　处：《福建医科大学学报》2011年第1期50-53,共4页Journal of Fujian Medical University

摘　　要：目的研究DNA结合/分化抑制蛋白-1(Id-1)在结直肠癌(CRC)中的表达,并通过分析其与碱性成纤维细胞生长因子(bFGF)、增殖细胞核抗原(PCNA)、Bcl-2的关系,探讨其在CRC发生发展过程中的意义。方法收集CRC手术切除标本103例,癌旁结直肠组织20例(离癌灶<3 cm),并以20例正常结直肠组织为对照,免疫组织化学方法检测其Id-1、bFGF、Bcl-2和PCNA的表达。结果 CRC中Id-1、bFGF、Bcl-2和PCNA的阳性表达率分别为80.6%(83/103)、78.6%(81/103)、70.9%(73/103)、81.6%(84/103),其阳性表达率和表达强度均明显高于正常结直肠组织。Id-1及bFGF的表达与结直肠癌的分化程度呈正相关。Id-1在≥5 cm的CRC中的表达高于<5 cm的CRC。Id-1的表达与CRC的淋巴结转移无相关性。Id-1的表达与bFGF、Bcl-2及PCNA呈正相关性(分别为r=0.372,P<0.001;r=0.274,P=0.005;r=0.303,P=0.002)。结论 Id-1在CRC中可能参与了肿瘤血管形成、抑制细胞凋亡的调控及细胞增殖调控,但可能不直接参与CRC的转移。Objective To study the relationship between the expression of inhibitor of differentiation/ DNA binding-1（Id-1） and the expressions of basic fibroblast growth factor（bFGF）,Bcl-2 and proliferating cell nuclear antigen（PCNA）,and the potential role of Id-1 in the carcinogenesis of colorectal cancer（CRC）.Methods A total of 103 cases of CRC specimens and 20 cases of adjacent non-tumor colorectal tissue were obtained,and 20 cases of normal colorectal tissue specimens were used for comparison.Immunohistochemical technique was applied to detect the expressions of Id-1,bFGF,Bcl-2 and PCNA.Results The positive expression rates of Id-1,bFGF,Bcl-2 and PCNA in tumor tissues were 80.6%,78.6%,70.9% and 81.6%,respectively.The positive expression rate and intensity were significantly higher than those in normal colorectal tissues.The expression of both Id-1 and bFGF was positively correlated with the histological grade.The positive rate of Id-1 was significantly correlated with the tumor size and was not correlated with the tumor metastasis.The expression of Id-1 was positively associated with bFGF,Bcl-2 and PCNA.Conclusion The expression of Id-1 may be involved in the vascularization of CRC,the regulation of apoptosis and proliferation of CRC cells.The results of the current study suggest that the increased expression of Id-1 may play an important role in CRC size but the expression of Id-1 is not associated with tumor metastasis.

关 键 词：结直肠肿瘤 分化抑制蛋白1 成纤维细胞生长因子2 增殖细胞核抗原 免疫组织化学 

分 类 号：R735.350.2[医药卫生—肿瘤] R735.370.2[医药卫生—临床医学]