牙周炎症对中晚期妊娠孕妇血清白细胞介素-10的影响  

Maternal periodontal inflammation and serum IL-10 in median or late pregnancy

作　　者：徐辉[1] 翁志强[2] 毛钊[1] 金磊[1] 尹英[1] 唐成忠[1] 李益材[1] 冷旭[1] 

机构地区：[1]南京军区南京总医院口腔科,南京口腔医学硕士210002 [2]南京军区南京总医院门诊部,南京210002

出　　处：《医学研究生学报》2011年第4期354-358,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金(30870722);南京军区南京总医院科研基金(2009M018)

摘　　要：目的母体牙周病与一些不良妊娠结局存在一定联系,其具体关联机制是目前研究的热点之一。文中拟从牙周病对孕妇全身炎性状态的影响,探讨牙周炎症对中晚期妊娠孕妇的血清炎性细胞因子白细胞介素-10(IL-10)的影响。方法根据牙周健康状况选择84例中晚期妊娠孕妇作为研究对象,并将其分为对照组(n=30)牙龈炎组(n=30)和牙周炎组(n=24),分别记录牙龈指数(ginginval index,GI)、探诊牙周袋(probing depth,PD)深度、临床附着丧失(clinical attachmentloss,CAL)、龈沟出血指数(sulcus bleeding index,SBI)和牙龈炎或牙周炎阳性位点率等。用Whatman I号滤纸条采集龈沟液(gingival crevicular fluid,GCF),并抽取空腹肘静脉血5 ml;ELISA法测定GCF和血清IL-10水平。结果牙龈炎组GI、PD、阳性位点率、GCF和血清IL-10与对照组比较,差异有显著性统计学意义(P<0.05);而牙周炎组与对照组和牙龈炎组间两两比较的结果,不仅在各牙周指数上差异显著(P<0.05),GCF和血清IL-10水平的差异也均有显著性统计学意义(P<0.05)。相关分析发现,GI、PD、CAL和阳性位点率分别与血清IL-10之间呈负相关(r=-0.333,-0.376,-0.322,-0.293)。回归分析发现,GCF的IL-10对血清IL-10的回归贡献较大(R2=0.71)。结论①牙龈炎和牙周炎与中晚期妊娠的血清IL-10水平下调存在显著关联。②GI、PD、CAL和阳性位点率分别与血清IL-10之间呈负相关。③GCF的IL-10对血清IL-10的回归贡献较大,表明局部牙周炎症与孕妇全身血清IL-10水平的表达减弱存在一定联系。Objective Maternal periodontal disease is associated with some adverse pregnancy outcomes,but its possible mechanisms are not yet fully understood.This study is to investigate the influence of the maternal periodontal status on the serum IL-10 level in median or late pregnancy.Methods Based on the periodontal health status,84 women in median or late pregnancy were divided into a periodontitis（PG,n=24）,a gingivitis（GG,n=30）,and a control group（CG,n=30）.Data were obtained concerning the gingival index（GI）,probing depth（PD）,clinical attachment loss（CAL）,sulcus bleeding index（SBI） and the positive sites of gingivitis or periodontitis of each participant.Gingival crevicular fluid（GCF） was gathered with filter paper strips,fasting peripheral blood samples collected by venipuncture,and GCF and serum IL-10 determined by ELISA.Results Statistically significant differences were found in GI,PD,SBI,rate of positive sites,GCF and serum IL-10 between GG and CG（P0.05）.Similar differences were observed between PG and GG as well as between PG and CG（P0.05）.The level of serum IL-10 was negatively correlated with GI,PD,CAL and the rate of positive sites（r =-0.333,-0.376,-0.322 and-0.293）.Regression analysis revealed a greater contribution of GCF IL-10 to serum IL-10（R2 = 0.71）.Conclusion Gingivitis and periodontitis are evidently associated with descended serum IL-10 in median and late pregnancy.The serum IL-10 level is negatively correlated with GI,PD,CAL and positive site rate.The increased regression contribution of GCF IL-10 to serum IL-10 shows a correlation of maternal local periodontal inflammation with the reduced expression of serum IL-10.

关 键 词：牙周炎 妊娠 炎性细胞因子 龈沟液 白细胞介素-10 

分 类 号：R783.3[医药卫生—口腔医学]