EN1调节UTROPHIN表达机制  

The regulation of UTROPHIN expression by EN1

作　　者：王谦[1,2] 曹东华[3] 林长坤[2] 王正东[2] 崔婉婷[2] 金春莲[2] 

机构地区：[1]中国医科大学高职学院,沈阳110001 [2]中国医科大学基础医学院医学遗传学教研室,沈阳110001 [3]中国人民解放军第二O二医院检验科,沈阳110003

出　　处：《遗传》2011年第4期347-352,共6页Hereditas（Beijing)

基　　金：国家十一五攻关课题项目(编号:2006BAI05A08)资助

摘　　要：为了探讨人类UTROPHIN蛋白表达上调的可能因素,文章利用P-match软件预测人类UTROPHIN基因启动子区域可能的调控元件,应用CHIP和EMSA实验加以验证,并利用RNA干扰和荧光定量PCR的方法分析人类EN1转录因子调节UTROPHIN表达的作用机制。经P-match软件预测,UTROPHIN基因启动子区域有2个转录因子EN1的可能结合位点,经CHIP和EMSA实验证实位点2是真正结合位点。通过RNA干扰实验,发现在HeLa细胞中EN1基因沉默后,可使UTROPHIN mRNA水平上调。以UTROPHIN蛋白为靶点可能为DMD(Duchenne muscular dystrophy)基因治疗提供一种新的策略。To investigate possible factors up-regulating the expression of UTROPHtN, potential regulatory elements in the promoter of the human UTROPH1,N was predicted by P-match software and verified by EMSA and CHIP. The mechanism of EN1 regulation of the human UTROPH1N expression was evaluated by RNA interference and real-time PCR analyses. Two potential EN1 binding sites in UTROPHIN promoter region were predicted by P-Match software but only the second site was verified to interact directly with EN1 by EMSA and CHIP. The results from RNA interference and real-time PCR showed that the mRNA level of UTROPHIN increased in HeLa cells after EN1 was knockdowned by siRNA. It indicated that ENI might be a negative regulatory factor for UTROPH1N. Our study suggested that UTROPHIN might be a new target for DMD therapy.

关 键 词：UTROPHIN蛋白 EN1转录因子 RNA干扰 荧光定量PCR 

分 类 号：R746[医药卫生—神经病学与精神病学]